10837059001

Lysozyme

from hen egg white

• Szinonimák: Mucopeptide N-acetylmuramoyl-hydrolase, Muramidase, lysozyme
• Enzyme Commission szám: 3.2.1.17 (BRENDA, IUBMB)
• UNSPSC kód: 12352204

Tulajdonságok

• biológiai forrás: chicken egg white
• Minőségi szint: 100
• Teszt: ≥95%
• Forma: solid
• specifikus aktivitás: >23000 U/mg (Shugar units)
• molekulatömeg: 14.4 kDa
• kiszerelés: pkg of 10 g
• gyártó/kereskedő neve: Roche
• optimális pH: 6.0-7.0
• Abszorpció: 3.9 at 280 nm (10 mg enzyme/ml)

Leírás

Általános leírás

Muramidase, Mucopeptide N-acetylmuramoyl-hydrolase

Alkalmazás

Lysozyme has been used for:

• Cell wall degradation
• Preparation of protoplasts
• Bacteriolysis
• Pharmacology (anti-inflammatory effect on mucous membrane; healing of tissue; protection against bleeding; normalization of mucus secretion; dissolution of pyrogens; potentiation of antibiotics)
• Food and drinks (flavor enhancer)
• Sample preparation prior to isolation of nucleic acids

Lysozyme has been used in the monitoring of transcription by real-time -PCR and sample preparation.

Biokémiai/fiziológiai hatások

Lysozyme hydrolytically cleaves the bonds between N-acetyl-D-glucosamine and N-acetylmuramic acid residues (GlcNAc β1-4Mur) in mucopolysaccharides and the glycan skeletons of the corresponding peptidoglycans (the latter are glycosaminoglycans linked to or crosslinked by peptide chains). Lysozyme effectively destroys the cell walls of bacteria.

Egység definíció

One Shugar unit is that quantity of enzyme in 1 ml of a suspension of Micrococcus luteus of pH 7.0 whose initial absorbance at a wavelength of 450 nm is 0.750 for a pathlength of 10 mm which causes the absorbance to decrease at the rate of 0.001 per minute, all measurements being carried out at a temperature of +25 °C.

Elkészítési megjegyzés

Working solution: Lysozyme hydrochloride is readily soluble in water and buffer solutions but insoluble in organic solvents.
Storage conditions (working solution): -15 to -25 °C
Aqueous solutions of the hydrochloride (2 mg/ml dist. water) can be stored for several days at 2 to 8° C or at -15 to -25° C for several weeks.

Feloldás

Prepare a stock solution at a concentration of 50 mg/ml in dist. water. Dispense into aliquots and store at -15 to -25 °C. Discard each aliquot after use.

Tárolás és stabilitás

Store at 2 to 8 °C. (Store dry!)

Egyéb megjegyzések

For life science research only. Not for use in diagnostic procedures.

Biztonsági adatok

• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 2
• Lobbanási pont (F): does not flash
• Lobbanási pont (C): does not flash