62971
Lysozyme from chicken egg white
powder (crystalline), 70000-140000 U/mg
Szinonimák:
Mucopeptide N-acetylmuramoylhydrolase, Muramidase
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
CAS-szám:
EC-szám:
MDL-szám:
UNSPSC kód:
12352204
NACRES:
NA.22
Javasolt termékek
biológiai forrás
chicken egg white
Minőségi szint
form
powder (crystalline)
specifikus aktivitás
70000-140000 U/mg
molekulatömeg
single-chain 14.3 kDa
Mr ~14600
technika/technikák
cell based assay: suitable
alkalmasság
suitable for cell lysis
UniProt elérési szám
alkalmazás(ok)
cell analysis
tárolási hőmérséklet
2-8°C
Géninformáció
chicken ... LYZ(396218)
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Alkalmazás
Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.
Biokémiai/fiziológiai hatások
Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.
The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).
The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).
Egység definíció
1 U corresponds to the amount of enzyme which decreases the absorbance at 450 nm by 0.001 per minute at pH 7.0 and 25°C (Micrococcus luteus, ATCC 4698, as substrate)
Figyelmeztetés
Danger
Figyelmeztető mondatok
Óvintézkedésre vonatkozó mondatok
Veszélyességi osztályok
Resp. Sens. 1
Tárolási osztály kódja
11 - Combustible Solids
WGK
WGK 3
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Egyéni védőeszköz
Eyeshields, Gloves, type N95 (US)
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