Összes fotó(3)
Fontos dokumentumok
NA2100
GenElute™ Bacterial Genomic DNA Kits
sufficient for 10 purifications
Szinonimák:
Bacterial Genomic DNA, Gen Elute
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(3)
About This Item
UNSPSC kód:
41105501
NACRES:
NA.55
Javasolt termékek
Related Categories
Általános leírás
The GenElute Bacterial Genomic DNA kit provides a simple and convenient way to isolate pure genomic DNA from gram-negative bacteria. For most gram-positive bacteria, the kit must be used in conjunction with the optional lysozyme (L4919) to effectively lyse the thick peptidoglycan cell walls. A Gram-Positive Lysis Solution is provided as a diluent for preparing the lysozyme stock solutions.
The kit combines the advantages of a silica-based system with a microspin format and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.
The kit combines the advantages of a silica-based system with a microspin format and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.
Alkalmazás
The purified bacterial genomic DNA is ready for downstream applications such as:
- restriction endonuclease digestions
- PCR
- Southern blots
- cloning
Tulajdonságok és előnyök
- Starting material: Up to 1.5 mL of culture
- Expected yield: Up to 20 μg
- Elution volume: 400 μl
- Time required: 70 - 120 min
- A260/A280 ratio: 1.6 - 1.9
- No phenol, chloroform, or ethanol precipitation required
Elv
The bacteria are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. The addition of ethanol causes the DNA to bind when the lysate is spun through a silica membrane into a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution.
The expected yield of genomic DNA will vary depending on the cell density of the bacterial culture and the bacterial species and strain used. DNA purified with the GenElute kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.
The expected yield of genomic DNA will vary depending on the cell density of the bacterial culture and the bacterial species and strain used. DNA purified with the GenElute kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.
Egyéb megjegyzések
For additional information, please see www.sigma-aldrich.com/genomicdna.
Jogi információk
GenElute is a trademark of Sigma-Aldrich Co. LLC
Kit Components Also Available Separately
Product No.
Leírás
Biztonsági adatlap
javasolt
Product No.
Leírás
Árazás
kapcsolódó termék
Product No.
Leírás
Árazás
Figyelmeztetés
Danger
Figyelmeztető mondatok
Óvintézkedésre vonatkozó mondatok
Veszélyességi osztályok
Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Célzott szervek
Central nervous system, Respiratory system
Tárolási osztály kódja
3 - Flammable liquids
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Protocols
GenElute™ Bacterial Genomic DNA Kit protocol describes a simple and convenient way for the isolation of pure genomic DNA from bacteria.
Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.
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