G1N350

GenElute^™ Mammalian Genomic DNA Miniprep Kits

sufficient for 350 purifications

• Szinonimák: Mammalian Genomic DNA Miniprep, animal gDNA prep, gDNA miniprep, mammalian gDNA extraction, mammalian gDNA isolation, mammalian gDNA prep, mammalian gDNA purification, silica mammalian gDNA prep, Gen Elute
• UNSPSC kód: 41105501
• NACRES: NA.55

Tulajdonságok

• használat: sufficient for 350 purifications
• Minőségi szint: 200
• technika/technikák: DNA purification: suitable
• tárolási hőmérséklet: 15-25°C

Leírás

Általános leírás

The GenElute Mammalian Genomic DNA Purification Kit provides a simple and convenient way to isolate pure, high molecular weight DNA from a variety of mammalian sources. These kits use a silica-based membrane, specially selected for genomic DNA purification, in a convenient spin column format.

Isolate pure genomic DNA from cultured cells, tissues (including rodent tails), and fresh whole blood or white blood cells. The kit combines the advantages of silica binding with a microspin format and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.

Alkalmazás

GenElute^™ Mammalian Genomic DNA Miniprep Kits has been used:

• to extract DNA from fin samples
• to extract the BSTUI restriction enzyme from the DNA
• to isolate DNA from the blood samples extracted from caudal fin
• to extract tissue and hair follicle DNA

The purified genomic DNA is ready for immediate use in downstream applications such as:

• restriction endonuclease digestions
• PCR
• Southern blots
• sequencing reactions
• cloning
• ligation

Tulajdonságok és előnyök

• Expected yield: 25 μg from 2 x 10⁶ cultured cells; 30 μg from 25 mg of tissue
• Elution volume: 200 - 400 μl
• Time required: 20 min after lysis
• A₂₆₀/A₂₈₀ ratio: 1.6 - 1.9
• Mechanical homogenization required: No

Elv

The starting materials are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. Addition of ethanol causes DNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution. The expected yields of genomic DNA will vary depending on the amount and type of starting material used. DNA purified with the kit has an A₂₆₀/A₂₈₀ ratio between 1.6 and 1.9 and can be up to 50 kb in length.

Egyéb megjegyzések

For additional information, please see www.sigma-aldrich.com/genomicdna.

Jogi információk

GenElute is a trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

• C2112 Column Preparation Solution
• P2308 Proteinase K from Tritirachium album, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology
• R6148 RNase A solution

Biztonsági adatok

• Piktogramok: GHS08,GHS05,GHS07,GHS09
• Figyelmeztetés: Danger
• Figyelmeztető mondatok: H290,H302,H315,H318,H334,H335,H410
• Óvintézkedésre vonatkozó mondatok: P234 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Veszélyességi osztályok: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Célzott szervek: Respiratory system
• Tárolási osztály kódja: 8A - Combustible corrosive hazardous materials
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable