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G1N10

Sigma-Aldrich

GenElute™ Mammalian Genomic DNA Miniprep Kits

sufficient for 10 purifications

Szinonimák:

Mammalian Genomic DNA Miniprep, Gen Elute

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About This Item

UNSPSC kód:
41105501
NACRES:
NA.55

használat

sufficient for 10 purifications

Minőségi szint

200

tárolási hőmérséklet

15-25°C

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Általános leírás

The GenElute Mammalian Genomic DNA Purification Kit provides a simple and convenient way to isolate pure, high molecular weight DNA from a variety of mammalian sources. These kits use a silica-based membrane, specially selected for genomic DNA purification, in a convenient spin column format.

Isolate pure genomic DNA from cultured cells, tissues (including rodent tails), and fresh whole blood or white blood cells. The kit combines the advantages of silica binding with a microspin format and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.

Alkalmazás

The purified genomic DNA is ready for immediate use in downstream applications such as:
  • restriction endonuclease digestions
  • PCR
  • Southern blots
  • sequencing reactions
  • cloning
  • ligation

Tulajdonságok és előnyök

  • Expected yield: 25 μg from 2 x 106 cultured cells; 30 μg from 25 mg of tissue
  • Elution volume: 200 - 400 μl
  • Time required: 20 min after lysis
  • A260/A280 ratio: 1.6 - 1.9
  • Mechanical homogenization required: No

Elv

The starting materials are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. Addition of ethanol causes DNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution. The expected yields of genomic DNA will vary depending on the amount and type of starting material used. DNA purified with the kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.

Egyéb megjegyzések

For additional information, please see www.sigma-aldrich.com/genomicdna.

Jogi információk

GenElute is a trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

Product No.
Leírás
Biztonsági adatlap
  • C2112Column Preparation SolutionBiztonsági adatlap
  • P2308Proteinase K from Tritirachium album, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biologyBiztonsági adatlap
  • R6148RNase A solutionBiztonsági adatlap

javasolt

Product No.
Leírás
Árazás

kapcsolódó termék

Product No.
Leírás
Árazás

A9434

Ammonium chloride, for molecular biology, suitable for cell culture, ≥99.5%

P2308

Proteinase K from Tritirachium album, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology

E1510

Ethidium bromide solution, BioReagent, for molecular biology, 10 mg/mL in H2O

A9539

Agarose, BioReagent, for molecular biology, low EEO

G1N70

GenElute™ Mammalian Genomic DNA Miniprep Kits, sufficient for 70 purifications

D9281

Lambda DNA EcoR I Hind III Digest, for DNA electrophoresis

L3287

Escort™ IV Transfection Reagent, Lipid reagent for transient and stable transfection of mammalian and insect cells.

T6400

Tris-Borate-EDTA buffer, 5× Concentrate

G1N350

GenElute™ Mammalian Genomic DNA Miniprep Kits, sufficient for 350 purifications

R6148

RNase A solution

C2112

Column Preparation Solution

93221-001

RNAstable®, Tube Kit, for room temp preservation of RNA

T0956

Sigma Universal Transfection Reagent, Polymer reagent for transfecting common mammalian cell lines

SRE0005

Proteinase K from Tritirachium album, Reagents designed and manufactured under current ISO 13485 certification.

Figyelmeztetés

Danger

Figyelmeztető mondatok

H290,H302,H315,H318,H334,H335,H410

Veszélyességi osztályok

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Célzott szervek

Respiratory system

Tárolási osztály kódja

8A - Combustible corrosive hazardous materials

Lobbanási pont (F)

483.8 °F

Lobbanási pont (C)

251 °C

Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

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Sigma-Aldrich

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Column Preparation Solution

Sigma-Aldrich

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Összes mutatása

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Mammalian Genomic DNA Miniprep Kit

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