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SRE0005

Sigma-Aldrich

Proteinase K from Tritirachium album

Reagents designed and manufactured under current ISO 13485 certification.

Szinonimák:

Endopeptidase K

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(2)

About This Item

CAS-szám:
39450-01-6
Enzyme Commission szám:
3.4.21.64 (BRENDA, IUBMB)
EC-szám:
254-457-8
MDL-szám:
MFCD00132129
UNSPSC kód:
12352204
NACRES:
NA.32

grade

for molecular biology

Minőségi szint

500

Forma

buffered aqueous glycerol solution

molekulatömeg

28.93 kDa

koncentráció

≥10 mg/mL protein
≥800 unit/mL

szennyeződések

≤500 pg/mg DNA (PicoGreen® assay)

alkalmazás(ok)

diagnostic assay manufacturing

idegen aktivitás

DNAse, RNAse, exonuclease, endonuclease, and nickase, none detected

kiszállítva

wet ice

tárolási hőmérséklet

2-8°C

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Alkalmazás

Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.
Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.
Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria
Determination of enzyme localization on membranes
Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.
Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.

Biokémiai/fiziológiai hatások

Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA.

Egység definíció

One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 μmole of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).

Jogi információk

PicoGreen is a registered trademark of Life Technologies

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kapcsolódó termék

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Árazás

Piktogramok

Health hazardExclamation mark
GHS08,GHS07

Figyelmeztetés

Danger

Figyelmeztető mondatok

H315,H319,H334,H335

Veszélyességi osztályok

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Célzott szervek

Respiratory system

Tárolási osztály kódja

10 - Combustible liquids

WGK

WGK 1

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Válasszon a legfrissebb verziók közül:

Analitikai tanúsítványok (COA)

Lot/Batch Number
117M4169V
0000316051
0000316049
0000316053
0000181686

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Dokumentumtár megtekintése

Nikol Jankovska et al.
Biomedicines, 10(3) (2022-03-26)
Creutzfeldt-Jakob disease (CJD), the most common human prion disorder, may occur as "pure" neurodegeneration with isolated prion deposits in the brain tissue; however, comorbid cases with different concomitant neurodegenerative diseases have been reported. This retrospective study examined correlations of clinical
Martin L Daus et al.
The Journal of biological chemistry, 288(49), 35068-35080 (2013-10-29)
The self-replicative conformation of misfolded prion proteins (PrP) is considered a major determinant for the seeding activity, infectiousness, and strain characteristics of prions in different host species. Prion-associated seeding activity, which converts cellular prion protein (PrP(C)) into Proteinase K-resistant, infectious
Jian Liu et al.
Applied and environmental microbiology, 79(18), 5593-5600 (2013-07-10)
Stenotrophomonas maltophilia is an important global opportunistic pathogen for which limited therapeutics are available because of the emergence of multidrug-resistant strains. A novel bacteriocin, maltocin P28, which is produced by S. maltophilia strain P28, may be the first identified phage
Nuria Gonzalez-Montalban et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 27(9), 3702-3710 (2013-06-05)
Interspecies prion transmission often leads to stable changes in physical and biological features of prion strains, a phenomenon referred to as a strain mutation. It remains unknown whether changes in the replication environment in the absence of changes in PrP
Gianluigi Zanusso et al.
The Journal of biological chemistry, 289(8), 4870-4881 (2014-01-09)
The role of the GPI-anchor in prion disease pathogenesis is still a challenging issue. In vitro studies have shown that anchorless cellular prion protein (PrP(C)) undergoes aberrant post-translational processing and metabolism. Moreover, transgenic (Tg) mice overexpressing anchorless PrP(C) develop a
View All Related Papers

Cikkek

Proteinase K: Introduction & Applications

Proteinase K aids in molecular biology applications by digesting structural proteins, removing nucleases, and isolating intact genomic DNA.

Proteinase K in Tissue Samples

Pro K aids in disrupting cell membranes for DNA release, crucial for downstream molecular biology techniques.

Guide on How to Use Proteinase K in Different Procedures

Guidelines on use of proteinase K, an enzyme commonly used to degrade proteins, and protect DNA and RNA from degradation in samples.

How Proteinase K Can Be Used in Blood DNA Extraction Applications

In blood DNA extraction, Proteinase K, an enzyme commonly used to degrade proteins, can help break down the cellular and nuclear membranes, releasing DNA from the cells that protect it from degradation and increase purity/yield making it more suitable for various molecular biology techniques.

Összes mutatása

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

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