D1054

Dihydrorhodamine 123

≥95% purity, powder

• Szinonimák: DHR 123; Methyl 2-(3,6-diamino-9H-xanthen-9-yl)benzoate; Benzoic acid, 2-(3,6-diamino-9H-xanthen-9-yl)-, methyl ester
• Tapasztalati képlet (Hill-képlet): C₂₁H₁₈N₂O₃
• CAS-szám: 109244-58-8
• Molekulatömeg: 346.38
• Beilstein: 5989965
• MDL-szám: MFCD00285712
• UNSPSC kód: 12171500
• PubChem Substance ID: 24893337
• NACRES: NA.47

Tulajdonságok

• Terméknév: Dihydrorhodamine 123, ≥95%
• Minőségi szint: 200
• Teszt: ≥95%
• Forma: powder
• technika/technikák: titration: suitable
• szín: pink
• oldhatóság: DMSO: >5 mg/mL
• alkalmazás(ok): diagnostic assay manufacturing; hematology; histology
• tárolási hőmérséklet: −20°C
• SMILES string: COC(=O)c1ccccc1C2c3ccc(N)cc3Oc4cc(N)ccc24
• InChI: 1S/C21H18N2O3/c1-25-21(24)15-5-3-2-4-14(15)20-16-8-6-12(22)10-18(16)26-19-11-13(23)7-9-17(19)20/h2-11,20H,22-23H2,1H3
• Nemzetközi kémiai azonosító kulcs: FNEZBBILNYNQGC-UHFFFAOYSA-N

Leírás

Általános leírás

Dihydrorhodamine 123 (DHR123) is an uncharged non-fluorescent dye and a derivative of rhodamine 123 (R123). Dihydrorhodamine 123 (DHR123) is oxidized to fluorescent R123 within cells in the presence of reactive oxygen species and it localizes in mitochondria. It is an analog of 2,7-Dichlorodihydrofluorescein (DCDHF), lipophilic and diffuses through cell membrane.

Alkalmazás

Dihydrorhodamine 123 (DHR) has been used for the detection of free intracellular radicals or ROS (reactive oxygen species) in head kidney-derived macrophages. It has also been used in DHR assay in red blood cells and in oxidative burst assay in neutrophils.

Biokémiai/fiziológiai hatások

Dihydrorhodamine 123 detects superoxide ions at the single-cell level and is used for probing neutrophils superoxide generation in a respiratory burst event. It is also used for measuring oxidative stress in macrophages and human spermatozoa.

Dihydrorhodamine 123 is used for the detection of ROS (reactive oxygen species). Presence of ROS oxidizes dihydrorhodamine 123 to the fluorescent derivative rhodamine 123. The reaction happens in the presence of a peroxidase or a similar catalyst.

Szubsztrátok

Peroxidase substrate

Biztonsági adatok

• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves, type N95 (US)