MAB360

Anti-Glial Fibrillary Acidic Protein Antibody

CHEMICON^®, mouse monoclonal, GA5

• Szinonimák: GFAP
• UNSPSC kód: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Tulajdonságok

• product name: Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5, ascites fluid, clone GA5, Chemicon^®
• biológiai forrás: mouse
• Minőségi szint: 100
• antitest forma: ascites fluid
• antitest terméktípus: primary antibodies
• klón: GA5, monoclonal
• faj reaktivitás: rat, human, mouse, rabbit, chicken, pig, bovine
• kiszerelés: antibody small pack of 25 μL
• gyártó/kereskedő neve: Chemicon^®
• technika/technikák: immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• izotípus: IgG1
• NCBI elérési szám: NM_002055.2
• UniProt elérési szám: P14136
• kiszállítva: ambient
• tárolási hőmérséklet: −20°C
• célzott transzláció utáni módosítás: unmodified
• Géninformáció: human ... GFAP(2670)

Leírás

Általános leírás

Glial fibrillary acidic protein is a class-III intermediate filament. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system.

Egyediség

Glial fibrillar acidic protein. On western blots of extracts from a human glioma cell line (U33CG/343MG), MAB360 recognizes a band at approximately 51 kDa corresponding to GFAP (Debus, 1983). By immunohistochemistry it recognizes astrocytes and Bergmann glia cells, glioma and glial cell derived tumors. Shows no cross-reactivity with vimentin.

Reactivity with other species has not been determined.

Immunogen

Purified GFAP from porcine spinal cord.

Alkalmazás

Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5 detects level of Glial Fibrillary Acidic Protein & has been published & validated for use in IC, IH, IH(P) & WB with more than 65 product citations.

Immunocytochemistry:
A previous lot was used on cultured mammalian cells.

Immunohistochemistry(paraffin):
1:400-1:800 dilution from a previous lot was used on alcohol-fixed paraffin embedded sections of rat brain (cerebrum or cerebellum) and human brain.

Immunoblotting: A 1:1000 dilution of a previous lot was used.

Optimal working dilutions must be determined by the end user

Minőség

Routinely evaluated by Western Blot on Mouse brain lysates.

Western Blot Analysis:
1:1000 dilution of this lot detected GFAP on 10 μg of Mouse brain lysates.

Cél megnevezése

~ 51 kDa

Kapcsolódás

Replaces: 04-1031; 04-1062

Fizikai forma

Unpurified mouse monoclonal IgG1 liquid in buffer containing 15 mM sodium azide.

Analízis megjegyzés

Control
Positive Control: Cultured astrocytes, brain tissue (white matter), spinal chord, retina

Egyéb megjegyzések

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Jogi információk

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Biztonsági adatok

• Tárolási osztály kódja: 10 - Combustible liquids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable