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ABN78

Sigma-Aldrich

Anti-NeuN Antibody

rabbit polyclonal

Szinonimák:

RNA binding protein fox-1 homolog 3, Fox-1 homolog C, Fox-3, Hexaribonucleotide-binding protein 3, NeuN, Neuronal nuclei

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(3)

About This Item

UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
klón:
polyclonal
application:
ICC
IF
IHC
WB
faj reaktivitás:
mouse, human, snail, rat
technika/technikák:
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
citations:
578

Terméknév

Anti-NeuN Antibody (rabbit), from rabbit, purified by affinity chromatography

biológiai forrás

rabbit

Minőségi szint

100

antitest forma

affinity isolated antibody

antitest terméktípus

primary antibodies

klón

polyclonal

tisztítva

affinity chromatography

faj reaktivitás

mouse, human, snail, rat

technika/technikák

immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable

NCBI elérési szám

NP_001034256

UniProt elérési szám

Q8BIF2

kiszállítva

wet ice

tárolási hőmérséklet

2-8°C

célzott transzláció utáni módosítás

unmodified

Géninformáció

human ... RBFOX3(146713)
mouse ... Rbfox3(52897)
rat ... Rbfox3(287847)

Általános leírás

RNA binding protein fox-1 homolog 3 also known as NeuN (Neuronal nuclei) is encoded by the Rbfox3 (also known as D11Bwg0517e, Hrnbp3) gene (Gene ID 52897) in murine species. The RNA-binding Fox (Rbfox) family of splicing factors is comprised of three members, Rbfox1 (Fox-1 or A2BP1), Rbfox2 (Fox-2 or RBM9), and Rbfox3 (Fox-3, HRNBP3 or NeuN). Rbfox proteins regulate splicing of many neuronal transcripts (pre-mRNAs) by binding the sequence (U)GCAUG in introns flanking alternative exons. A (U)GCAUG motif that lies downstream of the alternative exon generally promotes Rbfox-dependent exon inclusion, whereas an upstream motif will usually repress exon inclusion. Originally characterized as a marker of post-mitotic neurons and named neuronal nuclei (NeuN), Rbfox3 plays a role in promoting neuronal differentiation through alternative splicing of Numb pre-mRNA during brain development. Rbfox3-knockout in mice is reported to result in defective hippocampal gene expression, defects in synaptic transmission and plasticity in the dentate gyrus, as well as increased seizure susceptibility and decreased anxiety-related behaviors. Likewise, human RBFOX3 gene mutations have been linked to neurodevelopmental delay, cognitive impairments, autistic features, and epilepsy.

Egyediség

Anti NeuN antibody from rabbit is a polyclonal antibody that targets NeuN N-terminal region that ends at the start of the RNA recognition motif (RRM). The target region is present in all human, mouse, and rat spliced isoforms reported by UniProt (A6NFN3, Q8BIF2, D4A2H6, A0A0G2K2J4).

Immunogén

Epitope: N-terminus
GST-tagged recombinant mouse NeuN N-terrminal fragment.

Alkalmazás

Anti-NeuN (rabbit) Antibody, Cat. No. ABN78, is a highly specific rabbit polyclonal antibody that targets NeuN and has been tested in Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected NeuN in cultured embryonic E18 rat hippocampus neurons.

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected NeuN-positive neurons in human (cerebellum and cerebral cortex) and mouse (hippocampus) brain tissue sections.

Immunocytochemistry Analysis: A representative lots immunostained 4% paraformaldehyde-fixed neurons isolated from snail brain (Nikolić, L., et al. (2013). J. Exp. Biol. 216(Pt 18):3531-3541).

Immunofluorescence Analysis: Representative lots detected NeuN-positive neurons in frozen brain sections from 4% paraformaldehyde-perfused mice (Cherry, J.D., et al. (2015). J. Neuroinflammation. 12:203; Huang, B., et al. (2015). Neuron. 85(6):1212-1226; Ataka, K., et al. (2013). PLoS One. 8(11):e81744).

Immunofluorescence Analysis: A representative lots detected NeuN-positive neurons in formalin-fixed and paraffin-embedded amyotrophic lateral sclerosis (ALS) human brain tissue sections (Fratta, P., et al. (2015). Neurobiol. Aging. 36(1):546.e1-7).

Immunohistochemistry Analysis: A representative lot detected NeuN-positive neurons in rat brain tissue sections (Mendonça, M.C., et al. (2013).Toxins. 5(12):2572-2588).

Western Blotting Analysis: Representative lot detected NeuN in mouse and rat brain tissue lysates (Huang, B., et al. (2015). Neuron. 85(6):1212-1226; Mendonça, M.C., et al. (2013).Toxins. 5(12):2572-2588).

This rabbit polyclonal antibody is also avaiable as Alexa Fluor™ 488 (ABN78A4), biotin (ABN78B), and Cy3 (ABN78C3) conjugates for immunocytochemistry, immunofluorescence, immunohistochemistry applications.
Research Category
Neuroscience
Research Sub Category
Developmental Signaling

Minőség

Evaluated by Western Blotting in mouse brain nuclear extract.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected NeuN in 10 µg of mouse brain nuclear extract.

Cél megnevezése

~48/42 kDa observed. 33.87/35.12 kDa (human isoform 1/2), 40.61/39.05/35.64/34.07/35.63 kDa (mouse isoform 1/2/3/4/5), 40.59kDa (rat; UniProt D4A2H6), 34.05 kDa (rat; UniProt A0A0G2K2J4) calculated. Uncharacterized bands may be observed in some lysate(s).

Fizikai forma

Affinity purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Tárolás és stabilitás

Stable for 1 year at 2-8°C from date of receipt.

Analízis megjegyzés

Control
Mouse brain nuclear extract

Egyéb megjegyzések

Concentration: Please refer to lot specific datasheet.

Jogi információk

ALEXA FLUOR is a trademark of Life Technologies

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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javasolt

Product No.
Leírás
Árazás

Tárolási osztály kódja

12 - Non Combustible Liquids

WGK

WGK 1

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

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Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

Jeong-Hwi Cho et al.
Molecular medicine reports, 13(4), 2949-2956 (2016-03-05)
4-Hydroxy‑3-methoxybenzaldehyde (vanillin) and 4-hydroxybenzyl alcohol (4-HBA) are well‑known phenolic compounds, which possess various therapeutic properties and are widely found in a variety of plants. In the present study, the effects of vanillin and 4‑HBA were first investigated on cell proliferation
C9orf72 frontotemporal lobar degeneration is characterised by frequent neuronal sense and antisense RNA foci.
Mizielinska, S; Lashley, T; Norona, FE; Clayton, EL; Ridler, CE; Fratta, P; Isaacs, AM
Acta neuropathologica null
Ganglioside GD3 is required for neurogenesis and long-term maintenance of neural stem cells in the postnatal mouse brain.
Wang, J; Cheng, A; Wakade, C; Yu, RK
The Journal of Neuroscience null
Félix Leroy et al.
eLife, 3, doi:10-doi:10 (2014-10-15)
In amyotrophic lateral sclerosis (ALS) the large motoneurons that innervate the fast-contracting muscle fibers (F-type motoneurons) are vulnerable and degenerate in adulthood. In contrast, the small motoneurons that innervate the slow-contracting fibers (S-type motoneurons) are resistant and do not degenerate.
Fabio Carletti et al.
Frontiers in cellular neuroscience, 10, 29-29 (2016-02-24)
The involvement of microtubule dynamics on bioelectric activity of neurons and neurotransmission represents a fascinating target of research in the context of neural excitability. It has been reported that alteration of microtubule cytoskeleton can lead to profound modifications of neural
View All Related Papers

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