N4142

Anti-Neurofilament 200 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Szinonimák: NF200 Antibody - Anti-Neurofilament 200 antibody produced in rabbit, Nf200 Antibody, AntiNeurofilament heavy chain
• MDL-szám: MFCD02263444
• UNSPSC kód: 12352203
• NACRES: NA.41

Tulajdonságok

• biológiai forrás: rabbit
• Minőségi szint: 200
• konjugátum: unconjugated
• antitest forma: IgG fraction of antiserum
• antitest terméktípus: primary antibodies
• klón: polyclonal
• form: buffered aqueous solution
• molekulatömeg: antigen 200 kDa
• faj reaktivitás: bovine, wide range
• kiszerelés: antibody small pack of 25 μL
• fejlettebb validálás: independent ( Antibodies); Learn more about Antibody Enhanced Validation
• technika/technikák: immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:80 using animal cerebellum sections; microarray: suitable; western blot: suitable
• kiszállítva: dry ice
• tárolási hőmérséklet: −20°C
• célzott transzláció utáni módosítás: unmodified
• Géninformáció: human ... NEFH(4744) ; mouse ... Nefh(380684) ; rat ... Nefh(24587)

Leírás

Általános leírás

Neurofilament 200, also known as neurofilament heavy polypeptide (NEFH), is encoded by the gene mapped to human chromosome 22q12.2. The encoded protein has a molecular mass of 200kDa and belongs to the group of type IV intermediate filaments which are key constituents of the neuronal cytoskeleton. NEFH contains C-terminal motifs involved in the formation of sidearm projections which cross-link the neurofilaments.

Egyediség

Shows a wide species cross-reactivity. Reacts with neurofilaments in the central and peripheral nervous system and localizes the neurofilament polypeptide.

Immunogen

Neurofilament 200 from bovine spinal cord.

Alkalmazás

Anti-neurofilament 200 antibody produced in rabbit has been used for immunolabeling.
Anti-neurofilament 200 antibody produced in rabbit has been used:

• for detection of neurofilaments using immunohistological staining
• in human astrocytoma cell line
• in post-mortem brain sections
• in human cremaster muscles
• for detection of neurofilaments by immunofluorescence staining in lumbar spinal cord sections of mice

Biokémiai/fiziológiai hatások

Neurofilament 200 plays a vital role in cytoarchitecture organization. Downregulated expression of the gene has been observed in schizophrenia patients. Loss of gene expression is associated with the development of prostate carcinoma. Mutation in the gene leads to sporadic amyotrophic lateral sclerosis (ALS).
Neurofilament 200 assembly during development is critical for normal functioning of neuronal cells. Mutations in gene encoding neurofilaments is associated with neurological disorder called the Charcot–Marie–Tooth.

Fizikai forma

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Tárolás és stabilitás

Store at -20°C. For continuous use, the product may be stored at 2-8°C for up to one month. For extended storage, solution may be frozen in working aliquots at –20°C. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biztonsági adatok

• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: WGK 2
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable