AB5622
Anti-MAP2 Antibody
CHEMICON®, rabbit polyclonal
Szinonimák:
Anti-MAP2 antibody, microtubule-associated protein 2
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(7)
About This Item
UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Javasolt termékek
product name
Anti-Microtubule-Associated Protein 2 (MAP2) Antibody, Chemicon®, from rabbit
biológiai forrás
rabbit
Minőségi szint
antitest forma
saturated ammonium sulfate (SAS) precipitated
antitest terméktípus
primary antibodies
klón
polyclonal
faj reaktivitás
rat, mouse, human
gyártó/kereskedő neve
Chemicon®
technika/technikák
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
NCBI elérési szám
UniProt elérési szám
célzott transzláció utáni módosítás
unmodified
Géninformáció
human ... MAP2(4133)
mouse ... Map2(17756)
rat ... Map2(25595)
Általános leírás
The microtubule-associated protein 2 (MAP2) is an abundant neuronal cytoskeletal protein that binds to tubulin and stabilizes microtubules. (Herzog and Weber, 1978). MAP2 is essential for the development and maitenance of neuronal morphology (Matus, 1991). In neurons MAP2 occurs as three primary isoforms, the high molecular weight MAP2a, MAP2b, and the low molecular weight MAP2c, that result from alternative splicing of the MAP2 gene (Chung et al., 1996). The low molecular weight isoform, MAP2c, is expressed in developing brain and is down-regulated during brain maturation, whereas the high molecular weight MAP2b is expressed in both developing and adult brain. The MAP2a appears only after brain maturation (Tucker, 1990). All these forms bind to microtubules through a domain near the carboxyl terminus that contains either three or four similar repeats of a 31-amino-acid motif (Lewis et al., 1988). MAP2 together with MAP4 and tau proteins belong to the family of thermostable proteins associated with microtubules.
Egyediség
Anti-MAP2 antibody’s reactivity with other species has not been determined. Anti-MAP2 antibody is specific for Microtubule-associated protein 2 (MAP2). The antibody recognizes all MAP2 isoforms (MAP2A, MAP2B, MAP2C and MAP2D). It shows greatest immunoreactivity with the HMW-MAP2 (MAP2A and MAP2B).
Immunogen
Purified Microtubule-associated protein from rat brain.
Alkalmazás
Detect Microtubule-Associated Protein 2 (MAP2) using this Anti-Microtubule-Associated Protein 2 (MAP2) Antibody validated for use in ELISA, IC, IH, IH(P) & WB with more than 55 product citations.
Immunocytochemistry:
1:1,000 dilution of a previous lot was used on primary neurons.
Immunohistochemistry:
1:1,000 dilution on 4% paraformaldehyde / PBS fixed tissue sections.
Immunoblot:
1:2,000 dilution was used on a previous lot. Recognizes MAP2A (280-300kDa, and MAP2B (270-280KDa), and to a lesser extent MAP2C (70kDa) and MAP2D (70-75kDa).
ELISA:
greater than or equal to1:2,000 dilution of a previous lot was used.
Optimal working dilutions must be determined by the end user.
1:1,000 dilution of a previous lot was used on primary neurons.
Immunohistochemistry:
1:1,000 dilution on 4% paraformaldehyde / PBS fixed tissue sections.
Immunoblot:
1:2,000 dilution was used on a previous lot. Recognizes MAP2A (280-300kDa, and MAP2B (270-280KDa), and to a lesser extent MAP2C (70kDa) and MAP2D (70-75kDa).
ELISA:
greater than or equal to1:2,000 dilution of a previous lot was used.
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neuronal & Glial Markers
Neurofilament & Neuron Metabolism
Neuronal & Glial Markers
Neurofilament & Neuron Metabolism
Minőség
Immunohistochemistry(paraffin) Analysis:
MAP2 (cat. # AB5622) staining on Normal Cerebellum. Tissue pretreated with Citrate, pH 6.0. This lot of antibody was diluted to 1:500, using IHC-Select Detection with HRP-DAB. Immunoreactivity is seen as fiber-like- staining (brown) on microtubule.
Optimal Staining With TE Buffer, pH 9.0, Epitope Retrieval: Human Cerebellum
MAP2 (cat. # AB5622) staining on Normal Cerebellum. Tissue pretreated with Citrate, pH 6.0. This lot of antibody was diluted to 1:500, using IHC-Select Detection with HRP-DAB. Immunoreactivity is seen as fiber-like- staining (brown) on microtubule.
Optimal Staining With TE Buffer, pH 9.0, Epitope Retrieval: Human Cerebellum
Cél megnevezése
Approx. 280 kDa doublet (MAP2a and MAP2b) and approx. 70 kDa doublet (MAP2c) on SDS-PAGE.
Fizikai forma
Ammonium Sulfate Precipitation
Format: Purified
Purified rabbit polyclonal in buffer containing PBS with 0.1% sodium azide as a preservative.
Tárolás és stabilitás
Maintain at -20ºC in undiluted aliquots for up to 6 months after date of receipt. Avoid repeatedfreeze/thaw cycles.
Analízis megjegyzés
Control
Human brain tissue or human glioblastoma T98G cells
Human brain tissue or human glioblastoma T98G cells
Egyéb megjegyzések
Jogi információk
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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javasolt
Tárolási osztály kódja
12 - Non Combustible Liquids
WGK
nwg
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
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