At -80°C, Triton X-100 is generally stable, but the stability can depend on the specific buffer conditions and the concentration of the detergent. In most cases, Triton X-100 remains effective at low temperatures, but it is advisable to avoid repeated freeze-thaw cycles, as these can lead to changes in the detergent's properties and effectiveness. Please note that the shelf life after opening is dependent upon various factors which might affect the stability and, therefore, it is not guaranteed and needs to be determined by the end user.
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Wichtige Dokumente
X100
Triton™ X-100
Triton™ X-100
laboratory grade
Synonym(e):
4-(1,1,3,3-Tetramethylbutyl)-phenyl-polyethylenglykol, Alkylphenylpolyethylenglykol, Octylphenoxypolyethoxyethanol
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
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About This Item
Lineare Formel:
t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
CAS-Nummer:
Beilstein:
2315025
MDL-Nummer:
UNSPSC-Code:
12161900
PubChem Substanz-ID:
NACRES:
NA.21
Preise und Verfügbarkeit sind derzeit nicht verfügbar.
Empfohlene Produkte
Biologische Quelle
synthetic
Qualitätsniveau
Qualität
laboratory grade
Beschreibung
non-ionic
Form
liquid
mp (Schmelzpunkt)
6 °C (43 °F)
Übergangstemp.
cloud point 65 °C
pour point ~7 °C
Löslichkeit
water: 0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow
SMILES String
CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1
InChI
1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3
InChIKey
HNLXNOZHXNSSPN-UHFFFAOYSA-N
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Allgemeine Beschreibung
Triton™ X-100 ist ein gewöhnliches nicht-ionisches Tensid und Emulgiermittel, das oft in biochemischen Anwendungen zur Lösung von Proteinen eingesetzt wird. Es wird als relativ milde, nicht-denaturierende Reinigungslösung angesehen und ist in zahlreichen Referenzen als standardmäßig zugegebenes Reagens angegeben. Es wird zum Lysieren von Zellen zwecks Extraktion von Protein und zellulären Organellen verwendet. Es kann außerdem die lebende Zellmembran für eineTransfektion permeabilisieren.[1][2]
Anwendung
Triton™ X wird für folgende Anwendungen eingesetzt:
Biochem./physiol. Wirkung
Häufig eingesetztes nichtionisches Detergens zur Rückgewinnung von Membrankomponenten unter milden nicht-denaturierenden Bedingungen.
Leistungsmerkmale und Vorteile
- Ideal für Anwendungen in der Forschung der Zellbiologie und Biochemie
- Nichtionisches nichtdenaturierendes Reinigungsmittel
- Überlegen als Benetzungsmittel und Emulgator
- Sehr vielseitiges Tensid
Sonstige Hinweise
Füllen Sie bitte dieses Formular aus, um weitere Informationen zu unserem Sortiment an Biochemikalien zu erhalten.
Rechtliche Hinweise
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
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auch häufig zusammen mit diesem Produkt gekauft
Produkt-Nr.
Beschreibung
Preisangaben
Signalwort
Danger
H-Sätze
Gefahreneinstufungen
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2
Lagerklassenschlüssel
10 - Combustible liquids
WGK
WGK 3
Flammpunkt (°F)
483.8 °F - closed cup
Flammpunkt (°C)
251 °C - closed cup
Persönliche Schutzausrüstung
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter, type N95 (US)
Zulassungslistungen
Zulassungslistungen werden hauptsächlich für chemische Produkte erstellt. Für nicht-chemische Produkte können hier nur begrenzte Angaben gemacht werden. Kein Eintrag bedeutet, dass keine der Komponenten gelistet ist. Es liegt in der Verantwortung des Benutzers, die sichere und legale Verwendung des Produkts zu gewährleisten.
EU REACH SVHC Candidate List
CAS No.
EU REACH Annex XIV (Authorisation List)
CAS No.
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Protokolle
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How long is Triton X-100 stable at -80 degrees in buffer?
1 answer-
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If a sterilization of Triton X-100 or its dilutions (maximum 10% of Triton X-100 in the total volume) is needed to remove DNAse and RNAse, what's the best approach?
1 answer-
Autoclaving undiluted Triton X-100 is generally not recommended for sterilization. However, dilute solutions, up to 10%, may be autoclaved. At certain
concentrations, solutions may become cloudy, but dispersible above the cloud point. The solution will clarify with stirring upon cooling. Note that DNAse and RNAse-free products are currently available. Please see the links below to review these product options:
https://www.sigmaaldrich.com/product/sigma/t8787
https://www.sigmaaldrich.com/product/mm/648466
https://www.sigmaaldrich.com/product/sigma/93443Helpful?
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdfHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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What is the correct CAS number for Triton X-100, 9002-93-1 or 9036-19-5?
1 answer-
CAS 9036-19-5 is a generic CAS number and is correct for Triton® X-100. With this number, neither the substitution pattern of the aromatic ring (para, meta, ortho), nor the amount of the repeating units of the oxyethylene units is specified or described. Triton® X-100 with CAS 9002-93-1 is also correct but it defines only the para-substitution.
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Will heating Triton to reduce its viscosity have any negative impact on the product? Does heating negatively affect the activity of Triton?
1 answer-
Triton X-100 can be frozen, thawed, and used. However, caution is advised when heating it above 50°C, as discoloration may occur. This note indicates that excessive heating can lead to some form of change in Triton X-100. It would be reasonable to assume that the recommended working range is from ambient temperature to 50°C (or slightly lower). If your process requires heating the solution to temperatures higher than 50°C, it is recommended to add Triton X-100 only after the temperature has dropped below 50°C or lower. If you need to work at temperatures exceeding 50°C, it is advisable to conduct solution stability studies to assess potential damage or breakdown of Triton X-100.
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Is a concentration of 8x10^-5 mol/L of Triton X-100 harmful to metal (steel/aluminum) and silicon equipment when submerged in water?
1 answer-
The laboratory has not specifically tested the corrosive properties of Triton X-100 on metal. However, according to a publication titled "Triton X-100 and Sodium Lauryl Sulfate Surfactants as Environmentally Friendly Corrosion Inhibitors for Steel in 0.5 M H2SO4" in Advances in Analytical Chemistry, Triton X-100 appears to act as a corrosion inhibitor for steel.
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What are the NFPA hazard numbers for this?
1 answer-
With the implementation of the Globally Harmonized System (GHS) of hazard classification in our safety data sheets (SDS), we decided to no longer maintain the National Fire Protection Association (NFPA) information in section 15 of the SDS. Therefore, we no longer provide updates to the NFPA ratings. Kindly review the SDS available at this link: https://www.sigmaaldrich.com/US/en/sds/sial/x100?userType=anonymous
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Hi, Bought the X100-100ml but cant find what the concentration is. Is this 100%? Kind regards,
1 answer-
The product is a neat or pure liquid, the concentration is 100 %.
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What's the molar concentration of the TritonX 100?? How can I make a 50mM concentration from this??
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This product is supplied as a neat or pure liquid with a density of 1.07 g/mL and an average molecular weight of 625 g/mol. Assuming 100%, the molarity is 1.712 M. To make 1 L of a 50 mM solution from this, use 29.2 mL of the 1.712 M starting concentration.
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