Unfortunately, there is no direct correlation between linear pore size measurements and nominal pore ratings based on retention of globular proteins (MWCO). However, several publications are available describing the separation of QDs by size based on the membrane MWCO. Please see the link below to review an example of one such publication:
https://pmc.ncbi.nlm.nih.gov/articles/PMC8271461/
D9652
Dialyseschlauch, Cellulosemembran
avg. flat width 33 mm (1.3 in.)
Synonym(e):
Cellulose Dialysis Tubing, Cellulose Membrane, Dialysis Membrane
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100 FT
€ 156,00
€ 156,00
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100 FT
€ 156,00
About This Item
UNSPSC-Code:
41123100
NACRES:
NB.22
€ 156,00
Check Cart for Availability
Empfohlene Produkte
Mittlerer Durchm.
21 mm , when full
Mittlere (flache) Breite
33 mm (1.3 in.)
Kapazität
~110 mL/ft
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Allgemeine Beschreibung
Typische Molekulargewicht-Ausschlussgrenze: 14.000
Verpackung
In Rollen, getrocknet
Qualität
Kann mehr als nur Spuren von Glycerin und Schwefelverbindungen enthalten.
Angaben zur Herstellung
Das als Feuchthaltemittel enthaltene Glycerin kann durch Waschen der Schläuche für 3–4 Stunden unter fließendem Wasser entfernt werden. Schwefelverbindungen können durch die Behandlung der Schläuche mit einer 0,3%igen (w/v) Natriumsulfidlösung für 1 Minute bei 80 °C entfernt werden. Mit heißem Wasser (60 °C) für 2 Minuten waschen; anschließend mit einer 0,2%igen (v/v) Schwefelsäurelösung versauern und dann mit heißem Wasser abspülen, um die Säure zu entfernen. Diese Schläuche halten den Großteil der Proteine mit einem Molekulargewicht von 12.000 oder größer zurück.
Lagerklassenschlüssel
13 - Non Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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Analysenzertifikate (COA)
Lot/Batch Number
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Verwandter Inhalt
Dialysis Tubing
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What is the molecular weight cut off for the Dialysis tubing cellulose membrane avg. flat width 33 mm (1.3 in.) with product number D9652? Is this suitable for purification of Quantum Dots which have size range from 2-10nm.
1 answer-
Helpful?
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Which organic solvents can be used with these membranes?
1 answer-
Regenerated cellulose membranes are typically compatible with acids and alcohols. Organic solvent compatibility varies from compound to compound. However, the solvent concentration, temperature, and exposure time are factors to be considered. Please see the table below to review the general membrane compatibility with various solvents.
Helpful?
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how do I prepare the tubings before usage?
1 answer-
Instructions have been provided in the 'Preparation Note' in the 'DESCRIPTION' section.
-Wash the tubing in running water for 3-4 hours to remove glycerol included as a humectant.
-Treat the tubing with a 0.3% (w/v) solution of sodium sulfide at 80°C for 1 minute to remove sulfur compounds.
-Wash with hot water (60°C) for 2 minutes, followed by acidification with a 0.2% (v/v) solution of sulfuric acid, then rinse with hot water to remove the acid.
This tubing will retain most proteins of molecular weight 12,000 or greater.Helpful?
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