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Fontos dokumentumok

T9157

Sigma-Aldrich

Tryptose Phosphate Broth

suitable for insect cell culture

Szinonimák:

Tryptose Broth

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

UNSPSC kód:
41106507
NACRES:
NA.73

biológiai forrás

bovine milk

Minőségi szint

form

solid

technika/technikák

cell culture | insect: suitable

kiszállítva

ambient

tárolási hőmérséklet

room temp

Általános leírás

Tryptose Phosphate Broth (TPB) comprises pyrimidine based components and is crucial for pyrimidine synthesis and electron transport chain in cells. In chick embryo fibroblast, it alleviates chloramphenicol inhibition.

Alkalmazás

In addition to its use for the growth of fastidious micro-organisms, Tryptose Phospate Broth (TPB) has been studied as supplement for the preparation of media that supports vaccine production in BHK-21 cells and the growth of SF21 insect cells in high-density perfusion culture stirred-tank bioreactors.
Tryptose Phosphate Broth has been used as a supplement:
  • in Eagle′s minimum essential medium alpha for growing Vero cells
  • for Vero-CCL81 cells in porcine epidemic diarrhea coronavirus propagation
  • in Dulbecco modified Eagle medium in LR7cells for mouse hepatitis virus propagation
  • in Glasgow minimum essential medium (GMEM) for Madin-Darby canine kidney (MDCK) cells.

Komponensek

Tryptose Phosphate Broth (TPB) is composed of four components: Tryptose (20g/L); Dextrose (2g/L); NaCl (5g/L) and Disodium Phosphate (2.5g/L) typically adjusted to pH 7.3. The tryptose component is a peptone (mixture of amino acids and short peptides) derived by the mixed enzymatic hydrolysis (pancreatic enzymes) of the milk protein casein. This hydrolysate provides a source of amino acid based nutrients and survival factors that support the growth of fastidious micro-organisms such as Brucella, Streptococcus, and Neisseria; as well as eukaryotic cells such as insect and animal cells. Dextrose provides a fermentable carbohydrate that can be used by fastidious mico-organisms. Sodium chloride maintains the osmotic and ionic equilibrium and disodium phosphate provides the basic buffering capacity.

Tárolási osztály kódja

11 - Combustible Solids

WGK

WGK 3

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Egyéni védőeszköz

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges


Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

Már rendelkezik ezzel a termékkel?

Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

Identification and characterization of a proteolytically primed form of the murine coronavirus spike proteins after fusion with the target cell
Wicht O, et al.
Journal of Virology, 88(9), 4943-4952 (2014)
Phytochemical analysis and in-vitro screening of selected Indian medicinal plants for antiviral activity against highly pathogenic avian influenza virus
Sood R, et al.
Spatula DD, 3(3), 81-88 (2013)
Aminopeptidase N is not required for porcine epidemic diarrhea virus cell entry
Li W, et al.
Virus Research, 235, 6-13 (2017)
Azali Azlan et al.
PLoS neglected tropical diseases, 15(1), e0008351-e0008351 (2021-01-23)
The Asian tiger mosquito, Aedes albopictus (Ae. albopictus), is an important vector that transmits arboviruses such as dengue (DENV), Zika (ZIKV) and Chikungunya virus (CHIKV). Long noncoding RNAs (lncRNAs) are known to regulate various biological processes. Knowledge on Ae. albopictus
Tryptose phosphate broth improves Rickettsia felis replication in mammalian cells
Saisongkorh W, et al.
FEMS Immunology and Medical Microbiology, 64(1), 111-114 (2012)

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