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R7388

Sigma-Aldrich

RPMI-1640 Medium

With HEPES, with ʟ-glutamine, without sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Szinonimák:

Roswell Park Memorial Institute 1640 medium

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About This Item

MDL-szám:
MFCD00217820
UNSPSC kód:
12352207
NACRES:
NA.75

product name

RPMI-1640 Medium, Modified, with 20 mM HEPES and L-glutamine, without sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Minőségi szint

400

sterilitás

sterile-filtered

form

liquid

technika/technikák

cell culture | mammalian: suitable

szennyeződések

endotoxin, tested

pH

>7.2

alkatrészek

HEPES: 20 mM
NaHCO3: no
sodium pyruvate: no
phenol red: yes
L-glutamine: yes

kiszállítva

ambient

tárolási hőmérséklet

2-8°C

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Általános leírás

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.
RPMI-1640 medium formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. It is used for the culture of human normal and neoplastic leukocytes. RPMI-1640 also supports the growth of various types of cell cultures, like fresh human lymphocytes in the 72-hour phytohemagglutinin (PHA) stimulation assay.

Alkalmazás

RPMI-1640 medium has been used to culture:
  • human immortalized keratinocyte cells and in in vitro transfection assay
  • cell lines and human T-cell blasts and to monitor autophagic flux
  • peripheral blood mononuclear cells of piglets (PBMCs)

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kiegészítés

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Tárolási osztály kódja

12 - Non Combustible Liquids

WGK

WGK 1

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

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