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R8755

Sigma-Aldrich

RPMI-1640 Medium

With ʟ-glutamine, without phenol red and sodium bicarbonate, powder, suitable for cell culture

Szinonimák:

Roswell Park Memorial Institute 1640 medium

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Összes fotó(1)

About This Item

MDL-szám:
MFCD00217820
UNSPSC kód:
12352207
NACRES:
NA.75

Terméknév

RPMI-1640 Medium, Modified, with L-glutamine, without phenol red and sodium bicarbonate, powder, suitable for cell culture

Forma

powder

technika/technikák

cell culture | mammalian: suitable

alkatrészek

HEPES: no
phenol red: no
L-glutamine: yes
NaHCO3: no
sodium pyruvate: no

kiszállítva

ambient

tárolási hőmérséklet

2-8°C

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Általános leírás

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

Alkalmazás

RPMI-1640 Medium is used for the following applications:
  • Used in cell culture medium: Murine thymoma EL-4 cells were cultured in RPMI 1640 medium supplemented with other components
  • Used for the mycobacterial infection of splenocytes (in antibiotic-free RPMI 1640 medium)
  • Used for cell isolation and culture
  • Used in culture medium (as one of the component) during lymphocyte separation and culture, ELISPOT Assay, Ex Vivo Proliferation Assay
  • Used in medium for gp39 gene expression and CD40-Immunoglobuin binding assays
  • Used for the preparation of antifungal agents

Mennyiség

Formulated to contain 10.4 grams of powder per liter of medium.

Feloldás

Supplement with 2.0 g/L sodium bicarbonate.

Egyéb megjegyzések

Phenol red has been shown to interfere with the growth of some cells at clonal densities. Use this medium when working with stem cells or when growing cells at low densities. Also recommended for in vitro diagnostics use.

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Leírás
Árazás

kiegészítés

Product No.
Leírás
Árazás

Tárolási osztály kódja

11 - Combustible Solids

WGK

WGK 2

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Válasszon a legfrissebb verziók közül:

Analitikai tanúsítványok (COA)

Lot/Batch Number
0000361897
0000278220
0000361897
0000361709
0000275775

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Cikkek

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Az ELISpot assay minőségi és mennyiségi információt szolgáltat az immunválaszról, láthatóvá téve az egyes reagáló sejtekből származó többszörös szekréciós termékeket.

Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

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