M9281

3-Methyladenine

autophagy inhibitor

• Szinonimák: 3-MA, 6-Amino-3-methylpurine
• Tapasztalati képlet (Hill-képlet): C₆H₇N₅
• CAS-szám: 5142-23-4
• Molekulatömeg: 149.15
• Beilstein: 146087
• EC-szám: 225-908-6
• MDL-szám: MFCD00010531
• UNSPSC kód: 41106305
• PubChem Substance ID: 24897326
• NACRES: NA.51

Tulajdonságok

• biológiai forrás: synthetic (organic)
• Minőségi szint: 300
• Teszt: ≥99% (HPLC)
• Forma: powder
• mp: ~300 °C (dec.) (lit.)
• oldhatóság: DMF: 9.80-10.20 mg/mL, clear, colorless to light yellow
• SMILES string: Cn1cnc(N)c2ncnc12
• InChI: 1S/C6H7N5/c1-11-3-10-5(7)4-6(11)9-2-8-4/h2-3H,7H2,1H3
• Nemzetközi kémiai azonosító kulcs: FSASIHFSFGAIJM-UHFFFAOYSA-N

Leírás

Alkalmazás

3-Methyladenine (3-MA) is used to inhibit and study the mechanism of autophagy (lysosomal self-degradation) and apoptosis under various conditions. 3-MA inhibits autophagy by blocking autophagosome formation via the inhibition of type III Phosphatidylinositol 3-kinases (PI-3K). For use as an autophagy inhibitor, 3-MA is typically used at a concentration of 5 mM.

3-Methyladenine has been used as an autophagy inhibitor:

• to determine the expressions of autophagy-related genes (Beclin 1, light chain 3 (LC3) and p62) using the transfected human bone marrow mesenchymal stem cells (BM-MSCs)
• with cisplatin to study the inhibition of autophagy influenced cisplatin-induced apoptosis in A2780cp cells
• to study the interplay between colistin-induced autophagy and apoptosis

Standard for detection of nucleic acid methylation during carcinogenesis.

Biokémiai/fiziológiai hatások

3-Methyladenine (3-MA) is used to inhibit and study the mechanism of autophagy (lysosomal self-degradation) and apoptosis under various conditions. 3-MA inhibits autophagy by blocking autophagosome formation via the inhibition of type III phosphatidylinositol 3-kinases (PI-3K).

Elkészítési megjegyzés

This product is soluble in DMF (10 mg/mL; may require gentle heating). It is also soluble in water, 95% ethanol, or 1 N NaOH at 30 mg/mL with heating, but upon cooling, the product precipitates from solution. Solutions of 3-MA may be prepared in DMSO at 100 mM.

Egyéb megjegyzések

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Biztonsági adatok

• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable