L1668

Lectin from Phaseolus vulgaris (red kidney bean)

Phytohemagglutinin PHA-P, lyophilized powder (contains ~50% buffer salts as sodium phosphate and NaCl), BioReagent, suitable for cell culture

• Szinonimák: Phaseolus vulgaris agglutinin, PHA
• UNSPSC kód: 12352202
• NACRES: NA.32

Tulajdonságok

• Minőségi szint: 200
• termékcsalád: BioReagent
• Forma: lyophilized powder (contains ~50% buffer salts as sodium phosphate and NaCl)
• hatékonyság: ≤10 μg per mL mitogenic activity (Bromdeoxyuridine incorporation in human peripheral blood mononuclear cells)
• technika/technikák: cell culture | mammalian: suitable
• szín: white to off-white
• oldhatóság: PBS: 5 mg/mL, clear to slightly hazy, colorless to faintly yellow (pH 6.8)
• tárolási hőmérséklet: 2-8°C

Leírás

Alkalmazás

Lectin from Phaseolus vulgaris (red kidney bean) has been used:

• for the stimulation of cluster of differentiation (CD4+) T cells
• for the activation of primary peripheral blood lymphocytes
• for the stimulation of peripheral blood mononuclear cells

Biokémiai/fiziológiai hatások

PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.

Kiszerelés

Sold on the basis of lectin content

Analízis megjegyzés

Potency and solubility expressed in mg lectin/ml

Biztonsági adatok

• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves, type N95 (US)