L4144

Lectin from Phaseolus vulgaris (red kidney bean)

Leucoagglutinin PHA-L, lyophilized powder, BioReagent, suitable for cell culture

• Szinonimák: Phaseolus vulgaris agglutinin, PHA
• EC-szám: 232-718-7
• UNSPSC kód: 12352202
• NACRES: NA.32

Tulajdonságok

• Minőségi szint: 200
• termékcsalád: BioReagent
• form: lyophilized powder
• hatékonyság: erythroagglutination activity (Does not agglutinate a 2% suspension of human erythrocytes at 250 μg lectin/ml.); ≤1.0 μg per mL leucoagglutination activity; ≤5 μg per mL mitogenic activity (Bromdeoxyuridine incorporation in human peripheral blood mononuclear cells)
• technika/technikák: cell culture | mammalian: suitable
• szennyeződések: salt, free
• oldhatóság: DPBS: 5 mg/mL, slightly hazy, colorless
• alkalmasság: leucocyte agglutination activity tested
• tárolási hőmérséklet: 2-8°C

Leírás

Általános leírás

Lectins are carbohydrate-binding proteins, which contains four subunits with a molecular weight of 125 kDa. Leucoagglutinin PHA-L is a plant lectin, which is transported by the neurons in the central nervous system.

Alkalmazás

Lectin from Phaseolus vulgaris (red kidney bean) has been used:

• it has been used as a positive control to treat cells
• to stimulate cell culture for the analysis of lymphocyte proliferation
• to stimulate T cells

Biokémiai/fiziológiai hatások

Lectin agglutinates cells and precipitates polysaccharides and glycoproteins. It is associated with toxic allergens and hemagglutinins. Lectin exhibits anti-cancer, anti-human immunodeficiency virus (anti-HIV) and anti-microbial infection properties. Lectin inhibits mucosal atrophy, lowers type 2 diabetes and obesity, enhances nutrient absorption and regulates drug targeting.

PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.

Analízis megjegyzés

Agglutination activity is expressed in μg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of either human erythrocytes (2% in phosphate buffered saline, pH 6.8) or human leukocytes (10⁷ per mL in saline) after 1 hr incubation at 25 °C.

Biztonsági adatok

• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable