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Merck
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L8754

Sigma-Aldrich

Lectin from Phaseolus vulgaris (red kidney bean)

Phytohemagglutinin PHA-P, lyophilized powder

Szinonimák:

Phaseolus vulgaris agglutinin, PHA

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(2)

About This Item

EC-szám:
232-718-7
UNSPSC kód:
12352202
NACRES:
NA.32

biológiai forrás

Phaseolus vulgaris

Minőségi szint

300

Forma

lyophilized powder

hatékonyság

≤62.5 μg per mL agglutination activity
≤10 μg/mL mitogenic activity

szennyeződések

salt, free

tárolási hőmérséklet

2-8°C

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Általános leírás

PHAs are carbohydrate-binding proteins. There are several types of PHAs identified, predominantly in leguminous plants.
Phytohaemagglutinin (PHA, or phytohemagglutinin) is a lectin found in plants (esp. in legumes). It is obtained from Phaseolus vulgaris (red kidney beans) and acts as a mitogen in human leucocyte culture.

Alkalmazás

It has also been used as an antigen to measure in vivo T-cell-mediated immune responsiveness.
Lectin from Phaseolus vulgaris (red kidney bean) has been used in phytohemagglutinin (PHA) assay and during assessment of cellular and humoral immunity.

Biokémiai/fiziológiai hatások

PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.

Analízis megjegyzés

Agglutination activity is expressed in mg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of 2% human erythrocytes in phosphate buffered saline, pH 6.8, after 1 hr incubation at 25 °C. Mitogenic activity is determined by BrdU incorporation in HeLa cell cultures.

Tárolási osztály kódja

11 - Combustible Solids

WGK

WGK 3

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Válasszon a legfrissebb verziók közül:

Analitikai tanúsítványok (COA)

Lot/Batch Number
0000427537
0000427537
0000414307
0000414307
0000399491

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Dokumentumtár megtekintése

Laura Gangoso et al.
Oecologia, 178(4), 1113-1123 (2015-04-04)
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Conservation biology : the journal of the Society for Conservation Biology, 31(5), 1132-1140 (2017-01-12)
During the last decade, many studies have focused on the detrimental effects of noise pollution on acoustic communication. Surprisingly, although it is known that noise exposure strongly influences health in humans, studies on wildlife remain scarce. In order to gain
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The Journal of experimental biology, 223(Pt 20) (2020-09-13)
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A R S Badari Nath et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 134, 143-147 (2014-07-10)
Phytohaemagglutinin (PHA) is a lectin obtained from Phaseolus vulgaris (red kidney beans), that acts as a mitogen in human leucocyte culture and is commercially available from Gibco. This PHA (Gibco) was found to be very expensive, hence other inexpensive sources
Phytohemagglutinin content in fresh kidney bean in China
Sun Y, et al.
International journal of food properties, 22(1), 405-413 (2019)
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Cikkek

Lectin Selection Guide: A Useful Guide for Selecting the Right Lectin

Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

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