Összes fotó(1)
Fontos dokumentumok
11082132001
Roche
Phytohemagglutinin-M (PHA-M)
from Phaseolus vulgaris
Szinonimák:
PHA-M
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
23201100
Javasolt termékek
Minőségi szint
sterilitás
non-sterile; 0.2 μm filtered
Forma
lyophilized
kiszerelés
pkg of 20 mg
gyártó/kereskedő neve
Roche
alkalmazás(ok)
hematology
tárolási hőmérséklet
2-8°C
Általános leírás
Phytohemagglutinin (PHA), the lectin extract from the red kidney bean. PHA consists of five isolectins (L4E0, L3E1, L2E2, L1E3, L0E4) each being a tetramer held together by noncovalent forces. The subunits L (leukocyte reactive) have a high affinity for lymphocyte surface receptors, but little for those of erythrocytes, and are responsible for the mitogenic properties of the isolectins. E (erythrocyte reactive) is responsible for the erythrocyte-agglutinating properties.
Alkalmazás
Phytohemagglutinin (PHA) is a potent mitogen used to stimulate cell proliferation in lymphocyte cultures.
Phytohemagglutinin-M (PHA-M) has been used in the activation of Jurkat and primary cells. It has also been used to treat polymorphonuclear blood cells(PMBCs)
Biokémiai/fiziológiai hatások
Phytohemagglutinin (PHA) has mitogenic and potent cell agglutinating activities. It binds to T-cell membranes and stimulates cell division and metabolic activity. PHA has the ability to stimulate close contacts between cell membranes.
Specifikációk
Biological activity: <10 μg/ml for maximal stimulation of DNA synthesis (BrdU incorporation) with human peripheral blood lymphocytes.
Elkészítési megjegyzés
Working concentration: 2 - 10 μg/ml
Approximately 2 - 10 μg/ml for the stimulation of peripheral blood lymphocytes
Storage conditions (working solution): The reconstituted solution is stable for 2 weeks at 2 to 8 °C or for several months when stored in aliquots at -15 to -25 °C.
Approximately 2 - 10 μg/ml for the stimulation of peripheral blood lymphocytes
Storage conditions (working solution): The reconstituted solution is stable for 2 weeks at 2 to 8 °C or for several months when stored in aliquots at -15 to -25 °C.
Feloldás
PHA-M should be reconstituted in sterile double-dist. water (final concentration: 2–10 mg/ml). Further dilution with medium or PBS (phosphate buffered saline).
Egyéb megjegyzések
For life science research only. Not for use in diagnostic procedures.
Tárolási osztály kódja
11 - Combustible Solids
WGK
WGK 1
Lobbanási pont (F)
does not flash
Lobbanási pont (C)
does not flash
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