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L8777

Sigma-Aldrich

Lectin from Phytolacca americana (pokeweed)

lyophilized powder, BioReagent, suitable for cell culture

Szinonimák:

PWM, Pokeweed mitogen

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)

About This Item

MDL-szám:
MFCD00161973
UNSPSC kód:
12352202
NACRES:
NA.32

sterilitás

aseptically filled

Minőségi szint

200

termékcsalád

BioReagent

Forma

lyophilized powder

hatékonyság

≤0.3 μg/mL mitogenic activity

technika/technikák

cell culture | mammalian: suitable

oldhatóság

PBS: 1 mg/mL

tárolási hőmérséklet

2-8°C

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Általános leírás

Lectins are proteins of plant origin, which have the ability to bind to certain neuronal surface glycoproteins and glycolipids. This ability of theirs helps them to be used as neuroanatomical tracers, as they are taken and transported in a retrograde or anterograde fashion.

Alkalmazás

Lectin from Phytolacca americana (pokeweed) is used for proliferation Assay. (Cellular proliferative responses were measured by the incorporation of 3H thymidine in newly synthesized DNA, using a conventional assay).

Biokémiai/fiziológiai hatások

Lectins are carbohydrate-binding proteins and are highly specific for sugar moieties. Pokeweed lectin (PL) is a lectin specific for N-acetylglucosamine-containing saccharides that stimulate peripheral lymphocytes to undergo mitosis by binding to their cell surfaces. Four types of lectins are isolated from the roots of pokeweed (Phytolacca americana). They contain homologous domains but have different molecular sizes and biological properties.
PWM is extracted from pokeweed roots. This protein has hemagglutinating, leukoagglutinating, and mitogenic properties. PWM is not blood group specific, but has affinity for N-acetyl-β-D-glucosamine oligomers.

Minőség

Partially purified TCA precipitate.
Essentially salt-free.

Analízis megjegyzés

Mitogenic activity was determined by BrdU incorporation in HeLa cell cultures.

Piktogramok

Health hazard
GHS08

Figyelmeztetés

Danger

Figyelmeztető mondatok

H317,H334

Óvintézkedésre vonatkozó mondatok

P261 - P280 - P342 + P311

Veszélyességi osztályok

Resp. Sens. 1 - Skin Sens. 1

Tárolási osztály kódja

11 - Combustible Solids

WGK

WGK 3

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Egyéni védőeszköz

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Válasszon a legfrissebb verziók közül:

Analitikai tanúsítványok (COA)

Lot/Batch Number
0000400322
0000394021
0000377319
0000346865
0000346865

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Dokumentumtár megtekintése

Caroline Leeth et al.
Animals : an open access journal from MDPI, 11(10) (2021-10-24)
Immunological studies in the horse are frequently hampered by lack of environmental control, complicated study design, and ethical concerns when performing high risk studies. The purpose of the current study was to investigate the utility of a xenograft model for
Tomomi Fujii et al.
Acta crystallographica. Section D, Biological crystallography, 60(Pt 4), 665-673 (2004-03-25)
Pokeweed lectin (PL), a lectin specific for N-acetylglucosamine-containing saccharides, stimulates peripheral lymphocytes to undergo mitosis by binding to their cell surfaces. Four types of lectins have been isolated from the roots of pokeweed (Phytolacca americana) and shown to contain homologous
Kurt Hanevik et al.
The Journal of infectious diseases, 204(11), 1779-1786 (2011-10-13)
Clinical and epidemiological studies have suggested the development of acquired immunity in individuals previously infected with Giardia lamblia. However, there are no data on the long-term cellular immunity and genotype cross-reactivity. An outbreak of assemblage B giardiasis in a nonendemic
C R Gerfen et al.
Brain research, 290(2), 219-238 (1984-01-09)
A new neuroanatomical method for tracing connections in the central nervous system based on the anterograde axonal transport of the kidney bean lectin, Phaseolus vulgaris-leucoagglutinin (PHA-L) is described. The method, for which a detailed protocol is presented, offers several advantages
Samira Rezaei et al.
Scientific reports, 10(1), 7471-7471 (2020-05-06)
In the routine commercial karyotype analysis on 5,481 boars, we identified 32 carriers of mosaic reciprocal translocations, half of which were carrying a specific recurrent translocation, mos t(7;9). An additional 7 mosaic translocations were identified through lymphocyte karyotype analysis from
View All Related Papers

Cikkek

Lectin Selection Guide: A Useful Guide for Selecting the Right Lectin

Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

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