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Merck

G9422

Sigma-Aldrich

β-Glycerophosphate disodium salt hydrate

BioUltra, suitable for cell culture, suitable for plant cell culture, ≥99% (titration)

Szinonimák:

BGP, Glycerol 2-phosphate disodium salt hydrate

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

Lineáris képlet:
(HOCH2)2CHOP(O)(ONa)2 · xH2O
CAS-szám:
Molekulatömeg:
216.04 (anhydrous basis)
EC-szám:
MDL-szám:
UNSPSC kód:
12352201
PubChem Substance ID:
NACRES:
NA.25

biológiai forrás

synthetic (chemical)

Minőségi szint

termékcsalád

BioUltra

Teszt

≥99% (titration)

form

powder

technika/technikák

cell culture | mammalian: suitable
cell culture | plant: suitable

szennyeződések

≤0.1% Insoluble matter
≤0.5 mol % L-α-isomer

szín

white

mp

102-104 °C (lit.)

oldhatóság

water: soluble

anion nyomok

chloride (Cl-): ≤0.05%
sulfate (SO42-): ≤0.05%

kation nyomok

Al: ≤0.001%
Ca: ≤0.001%
Cu: ≤0.0005%
Fe: ≤0.001%
K: ≤0.005%
Mg: ≤0.0005%
Pb: ≤0.001%
Zn: ≤0.0005%

SMILES string

O.[Na+].[Na+].OCC(CO)OP([O-])([O-])=O

InChI

1S/C3H9O6P.2Na.H2O/c4-1-3(2-5)9-10(6,7)8;;;/h3-5H,1-2H2,(H2,6,7,8);;;1H2/q;2*+1;/p-2

Nemzetközi kémiai azonosító kulcs

ROPZSVKNEIIIDE-UHFFFAOYSA-L

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Általános leírás

Glycerophosphoric acid disodium salt hydrate, also known as BGP, is a versatile compound with applications across various research domains, including cell biology, biochemical research, and metabolomics. Recognized as an endogenous metabolite and a serine-threonine phosphatase inhibitor, BGP is extensively utilized in chitosan hydrogel formulations for drug delivery research. It serves as a phosphate source for cell growth and recombinant protein production, offering advantages in media formulations by reducing precipitation at higher pH levels. Additionally, BGP finds applications in micro-arc oxidation electrolyte solutions, contributing to the creation of photocatalytic coatings.
In cell biology, β-Glycerophosphate disodium salt plays a pivotal role as a phosphate group donor in matrix mineralization studies and accelerates calcification in vascular smooth muscle cells. It also promotes bone matrix mineralization when delivered to osteoblasts, providing a crucial source of phosphate ions. In the realm of biochemical research, BGP functions as a classical serine-threonine phosphatase inhibitor in kinase reaction buffers, offering broad-spectrum inhibition. Its role as an organic phosphate donor is particularly valuable in culture media for mesenchymal stem cell differentiation to osteoblast-type cells. Moreover, BGP is utilized to buffer M17 media for Lactococcus culture in recombinant protein expression. The comprehensive utility of BGP spans multiple areas of research, making it an essential component in studies encompassing cell biology, biochemical research, and metabolomics.

Alkalmazás

β-Glycerophosphate is a classical serine-threonine phosphatase inhibitor used in kinase reaction buffers. BGP is often used in combination with other phosphatase/protease inhibitors for broad spectrum inhibition. It functions as an organic phosphate donor and has been used in culture media for mesenchymal stem cell differentiation to osteoblast-type cells. BGP is also used to buffer M17 media for Lactococcus culture in recombinant protein expression.
Initiates differentiation of bone-marrow-derived stem cells embedded in a natural collagen/chitosan gel designed for tissue engineering.

Tulajdonságok és előnyök

  • Versatile and adaptable for a wide variety of laboratory and research applications
  • Suitable for Cell culture and Plant cell culture
  • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
  • BioUltra Grade for your Cell Biology and Biochemical research

Minőség

Low inorganic phosphate content. Mixtures of α- and β-glycerophosphate have historically been used in microbiological media but high levels of inorganic (free) phosphate has been associated with the irregular growth of bacteria and unwanted precipitates.

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Figyelmeztetés

Warning

Figyelmeztető mondatok

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Veszélyességi osztályok

Eye Irrit. 2

Tárolási osztály kódja

11 - Combustible Solids

WGK

WGK 3

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable


Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

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Dokumentumtár megtekintése

J Douglas et al.
The Journal of applied bacteriology, 56(2), 321-326 (1984-04-01)
An easily prepared medium, originally designed for the cultivation of lactic phages, has been found to have much wider application. Experience in its use over a ten year period with a range of physiologically diverse bacteria, for teaching and research
A Herrera et al.
Materials science & engineering. C, Materials for biological applications, 103, 109760-109760 (2019-07-28)
In the design of macroporous biomaterial scaffolds, attention is payed predominantly to the readily accessible macroscopic mechanical properties rather than to the mechanical properties experienced by the cells adhering to the material. However, the direct cell mechanical environment has been
Anna Di Vito et al.
Cell transplantation, 28(1), 129-139 (2018-10-30)
The periodontal ligament displays a reservoir of mesenchymal stem cells which can account for periodontal regeneration. Despite the numerous studies directed at the definition of optimal culture conditions for long-term expansion of periodontal ligament stem cells (PDLSCs), no consensus has
Kai-Chiang Yang et al.
Biotechnology and bioengineering, 115(9), 2341-2355 (2018-05-20)
Transplantation of encapsulated porcine islets is proposed to treat type 1 diabetes. However, the envelopment of fibrous tissue and the infiltration of immune cells impair islet function and eventually cause implant failure. It is known that hemodialysis using an ethylene
Lucas R Brun et al.
SpringerPlus, 3, 676-676 (2014-12-19)
The aim of this study was to evaluate the effect of strontium ranelate (SrR) on bone mineral density (BMD) and bone turnover markers after 1 year of treatment. Additionally, the effect of SrR in bisphosphonate-naïve patients (BP-naïve) compared to patients previously

Protocols

TrueGel3D® Hydrogel Plate protocol guides high-throughput culture of human adipose MSCs for screening applications.

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