P7626

Phenylmethanesulfonyl fluoride

powder, ≥98.5% (GC)

• Szinonimák: α-Toluenesulfonyl fluoride, Benzylsulfonyl fluoride, PMSF, Phenylmethylsulfonyl fluoride
• Tapasztalati képlet (Hill-képlet): C₇H₇FO₂S
• CAS-szám: 329-98-6
• Molekulatömeg: 174.19
• Beilstein: 2088311
• EC-szám: 206-350-2
• MDL-szám: MFCD00007424
• UNSPSC kód: 12352202
• PubChem Substance ID: 24898863
• NACRES: NA.77

Tulajdonságok

• Terméknév: Phenylmethanesulfonyl fluoride, ≥98.5% (GC)
• biológiai forrás: synthetic
• Minőségi szint: 300
• Teszt: ≥98.5% (GC)
• Forma: powder
• mp: 91-94 °C
• oldhatóság: dry solvents (ethanol, methanol, and 2-propanol): 200 mM (Stock solution are stable for months at 4°C.); H₂O: unstable
• SMILES string: FS(=O)(=O)Cc1ccccc1
• InChI: 1S/C7H7FO2S/c8-11(9,10)6-7-4-2-1-3-5-7/h1-5H,6H2
• Nemzetközi kémiai azonosító kulcs: YBYRMVIVWMBXKQ-UHFFFAOYSA-N

Leírás

Általános leírás

Phenylmethanesulfonyl fluoride (PMSF) is a widely used serine protease inhibitor, effective against such enzymes as chymotrypsin, thrombin, and trypsin. PMSF acts as an inhibitor via sulfonation of the hydroxyl residues of serine residues at the reactive sites of serine proteases.

Alkalmazás

PMSF is often used in lysis buffer to assist in preserving proteins of interest during protein isolation and sample preparation, by inhibiting proteases that would otherwise degrade proteins after cell or tissue lysis. PMSF is unstable in aqueous media. Thus stock solutions of PMSF are generally prepared in anhydrous organic solvents (e.g. 100% ethanol, or anhydrous isopropanol) prior to use in aqueous media.

Noted general features and benefits of PMSF include the following:

• Inhibits serine proteases such as trypsin and chymotrypsin
• Also inhibits cysteine proteases (reversible by reduced thiols) and mammalian acetylcholinesterase
• Not as effective or as toxic as DFP
• Effective concentration 0.1-1 mM
• Half-life = 1 hr. at pH 7.5

Phenylmethanesulfonyl fluoride has been used in following applications:

• cell fractionation.
• used as a supplement in nuclear protein extraction.
• inhibitor of cholesterol esterase (CE) and pseudocholinesterase (PCE).
• used for the collection of blood prior to centrifugation to quantify plasma ANP levels.

Biokémiai/fiziológiai hatások

Administration of PMSF produces analgesia unrelated to its anticholinesterase effect, and prolongs the analagesic effect of centrally administered β-endorphin.

Egyéb megjegyzések

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Biztonsági adatok

• Piktogramok: GHS06,GHS05
• Figyelmeztetés: Danger
• Figyelmeztető mondatok: H301,H314
• Óvintézkedésre vonatkozó mondatok: P260 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363
• Veszélyességi osztályok: Acute Tox. 3 Oral - Skin Corr. 1B
• Tárolási osztály kódja: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges