C4879

α-Chymotrypsinogen A from bovine pancreas

essentially salt-free, lyophilized powder

• Szinonimák: chymotrypsin A zymogen
• CAS-szám: 9035-75-0
• EC-szám: 232-905-3
• MDL-szám: MFCD00130483
• UNSPSC kód: 12352204
• NACRES: NA.54

Tulajdonságok

• biológiai forrás: bovine pancreas
• Minőségi szint: 200
• típus: Type II
• form: essentially salt-free, lyophilized powder
• specifikus aktivitás: ≥40 units/mg solid
• molekulatömeg: 25,656 Da by calculation
• tisztítva: 6× crystallization
• oldhatóság: 1 mM HCl: soluble 10 mg/mL, clear, colorless
• UniProt elérési szám: P00767
• idegen aktivitás: α-chymotrypsin ≤1 U/mg (prior to activation by trypsin)
• tárolási hőmérséklet: −20°C
• Géninformáció: cow ... CTRB1(618826)

Leírás

Általános leírás

Chymotrypsinogen from bovine pancreas is a zymogen containing 5 disulfide bridges. It has an isoelectric pH of 8.97.

Alkalmazás

α-Chymotrypsinogen A from bovine pancreas has been used as model protein crystallization reproducibility studies. It has also been used in the hydrolysis of α-gliadins prior to mass spectroscopy studies.

The enzyme from Sigma has been used in the non-invasive determination of solid-state protein conformation using near infrared (NIR) spectroscopy. It has been used to study the partitioning of protein in polymer/polymer aqueous two-phase systems. The enzyme has also been used for self-interaction chromatography applications, to test the rapid measurement of protein osmotic second virial coefficients. In this technique, the protein is immobilized on chromatographic particles and its retention is measured using isocratic elution.

Biokémiai/fiziológiai hatások

Chymotrypsinogen A requires limited proteolysis for its activation. Chymotrypsinogen A may be activated by trypsin and chymotrypsin (autolytic activation) to form m α, β, γ, δ and π chymotrypsin (depending upon the conditions of activation). Chymotrypsin is a protease that will preferentially cleave peptides on the carboxyl side of aromatic amino acids including tryptophan, tyrosine, and phenylalanine. It will also hydrolyze peptides on the carboxyl side of leucine, methionine, and alanine.

A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met) on the carboxyl end of the peptide bond.

Egység definíció

After activation to Chymotrypsin, one unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.

Egyéb megjegyzések

View more information on chymotrypsin at www.sigma-aldrich.com/enzymeexplorer

Biztonsági adatok

• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves, type N95 (US)