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C3260

Sigma-Aldrich

Citrate Synthase from porcine heart

ammonium sulfate suspension, ≥100 units/mg protein

Szinonimák:

Citrate condensing enzyme, Citrate oxaloacetate lyase (CoA-acetylating)

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About This Item

CAS-szám:
9027-96-7
Enzyme Commission szám:
4.1.3.7 (BRENDA, IUBMB)
MDL-szám:
MFCD00130808
UNSPSC kód:
12352204
NACRES:
NA.54

biológiai forrás

Porcine heart

Minőségi szint

200

form

ammonium sulfate suspension

specifikus aktivitás

≥100 units/mg protein

molekulatömeg

98 kDa ( 49 kDa monomer)

oldhatóság

H2O: soluble 1.0 mg/mL, clear

idegen aktivitás

isocitrate dehydrogenase and aconitase ≤0.01%
malic dehydrogenase ≤0.1%

tárolási hőmérséklet

2-8°C

Alkalmazás

Citrate Synthase from porcine heart has been used:
  • to inject newt egg for determining its importance in egg activation at fertilization
  • in the preparation of reaction mix to determine pyruvate carboxylase enzyme activity
  • to examine whether it can induce a [Ca2+] increase and egg activation in unfertilized eggs

Biokémiai/fiziológiai hatások

Citrate synthase catalyses the conversion of citrate to acetyl-CoA in the presence of coenzyme-A with the release of H2O and oxaloacetate. The enzyme has a molecular weight of 85 kDa and a pI of 6.1-6.6. It is inhibited by fluoroacetyl-CoA, palmitoyl-CoA, and citroyl-CoA. It is also inhibited when it is acetylated by acetic anhydride or iodinated by iodine.

Egység definíció

One unit will form 1.0 μmole of citrate from oxalacetate and acetyl CoA per min at pH 8.0 at 37 °C.

Fizikai forma

Suspension in 3.2 M (NH4)2SO4 solution, pH 7.0.

Elkészítési megjegyzés

Dissolves in water to form a clear solution at 1 mg/mL concentration.

Tárolási osztály kódja

12 - Non Combustible Liquids

WGK

WGK 1

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

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Dokumentumtár megtekintése

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Cikkek

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

Hogyan dolgozzunk az ammónium-szulfát szuszpenzióként szállított enzimekkel?

Az ammónium-szulfát szuszpenzióként szállított enzimekkel való munkavégzésre vonatkozó utasítások

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

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