Przejdź do zawartości
Merck

Osteoblast biocompatibility of premineralized, hexamethylene-1,6-diaminocarboxysulfonate crosslinked chitosan fibers.

Journal of biomedical materials research. Part A (2015-03-17)
Marjorie A Kiechel, Laura T Beringer, Amalie E Donius, Yuko Komiya, Raymond Habas, Ulrike G K Wegst, Caroline L Schauer
ABSTRAKT

Biopolymer-ceramic composites are thought to be particularly promising materials for bone tissue engineering as they more closely mimic natural bone. Here, we demonstrate the fabrication by electrospinning of fibrous chitosan-hydroxyapatite composite scaffolds with low (1 wt %) and high (10 wt %) mineral contents. Scanning electron microscopy (FESEM), energy dispersive spectroscopy (EDS) and unidirectional tensile testing were performed to determine fiber surface morphology, elemental composition, and tensile Young's modulus (E) and ultimate tensile strength (σUTS ), respectively. EDS scans of the scaffolds indicated that the fibers, crosslinked with either hexamethylene-1,6-diaminocarboxysulfonate (HDACS) or genipin, have a crystalline hydroxyapatite mineral content at 10 wt % additive. Moreover, FESEM micrographs showed that all electrospun fibers have diameters (122-249 nm), which fall within the range of those of fibrous collagen found in the extracellular matrix of bone. Young's modulus and ultimate tensile strength of the various crosslinked composite compositions were in the range of 116-329 MPa and 2-15 MPa, respectively. Osteocytes seeded onto the mineralized fibers were able to demonstrate good biocompatibility enhancing the potential use for this material in future bone tissue engineering applications.

MATERIAŁY
Numer produktu
Marka
Opis produktu

Sigma-Aldrich
Carbon nanofibers, graphitized, platelets(conical), >98% carbon basis, D × L 100 nm × 20-200 μm
Sigma-Aldrich
Carbon nanofibers, pyrolitically stripped, platelets(conical), >98% carbon basis, D × L 100 nm × 20-200 μm
Sigma-Aldrich
N,O-Bis(trimethylsilyl)acetamide, synthesis grade, ≥95%
Sigma-Aldrich
Methane-12C, 13C-depleted, 99.9 atom % 12C
Sigma-Aldrich
Sodium phosphate, 96%
Sigma-Aldrich
DAPI, for nucleic acid staining
Sigma-Aldrich
L-Glutamine, ReagentPlus®, ≥99% (HPLC)
Sigma-Aldrich
L-Glutamine
Sigma-Aldrich
L-Glutamine, BioUltra, ≥99.5% (NT)
SAFC
L-Glutamine
Sigma-Aldrich
L-Glutamine, meets USP testing specifications, suitable for cell culture, 99.0-101.0%, from non-animal source
Sigma-Aldrich
L-Glutamine, γ-irradiated, BioXtra, suitable for cell culture
Węgiel - Szklisty, rod, 100mm, diameter 5.0mm, glassy carbon
Węgiel - Szklisty, foil, 50x50mm, thickness 1.0mm, glassy carbon
Węgiel - Szklisty, foil, 100x100mm, thickness 1.0mm, glassy carbon
Węgiel - Szklisty, rod, 100mm, diameter 7.0mm, glassy carbon
Węgiel - Szklisty, rod, 200mm, diameter 7.0mm, glassy carbon
Węgiel - Szklisty, rod, 200mm, diameter 1.0mm, glassy carbon
Węgiel - Szklisty, foil, 100x100mm, thickness 2.0mm, glassy carbon
Węgiel - Szklisty, rod, 50mm, diameter 1.0mm, glassy carbon
Węgiel - Szklisty, rod, 200mm, diameter 10mm, glassy carbon
Węgiel - Szklisty, foil, 100x100mm, thickness 6.0mm, glassy carbon
Węgiel - Szklisty, rod, 5mm, diameter 3.0mm, glassy carbon
Węgiel - Szklisty, rod, 100mm, diameter 1.0mm, glassy carbon
Węgiel - Szklisty, rod, 200mm, diameter 3.0mm, glassy carbon
Węgiel - Szklisty, foam, 150x150mm, thickness 2.5mm, bulk density 0.05g/cm3, porosity 96.5%
Węgiel - Szklisty, foil, 10x10mm, thickness 1.0mm, glassy carbon
Węgiel - Szklisty, foam, 150x150mm, thickness 3.2mm, bulk density 0.05g/cm3, porosity 96.5%
Węgiel - Szklisty, rod, 200mm, diameter 5.0mm, glassy carbon
Węgiel - Szklisty, foam, 300x300mm, thickness 30mm, bulk density 0.05g/cm3, porosity 96.5%