Ugrás a tartalomra
Merck
Összes fotó(6)

Fontos dokumentumok

View All Documentation

V2258

Sigma-Aldrich

Monoclonal Anti-Vimentin antibody produced in mouse

clone LN-6, ascites fluid

Szinonimák:

Anti-F5H288

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(6)

About This Item

MDL-szám:
MFCD00162904
UNSPSC kód:
12352203
NACRES:
NA.41

biológiai forrás

mouse

Minőségi szint

200

konjugátum

unconjugated

antitest forma

ascites fluid

antitest terméktípus

primary antibodies

klón

LN-6, monoclonal

tartalmaz

15 mM sodium azide

faj reaktivitás

pig, mouse, sheep, human, bovine, rabbit, feline, rat

technika/technikák

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using human tissue sections
immunoprecipitation (IP): suitable
indirect immunofluorescence: suitable using cultured cells
western blot: suitable

izotípus

IgM

UniProt elérési szám

P08670

kiszállítva

dry ice

tárolási hőmérséklet

−20°C

célzott transzláció utáni módosítás

unmodified

Géninformáció

human ... VIM(7431)
mouse ... Vim(22352)
rat ... Vim(81818)

Általános leírás

Vimentin belongs to the class of type III intermediate filament protein family. The gene is located on human chromosome 10p13. It is a cytoskeletal protein. Vimentin is mainly expressed in normal mesenchymal cells.
Vimentin is a 58 kD class III intermediate filament present mainly in mesenchymal cells. It regulates cell shape, motility and endurance of mechanical stress in mesenchymal cells. Monoclonal anti-vimentin antibody can be used for the localization of vimentin by various immunochemical assays like immunoblotting, immunohistochemistry and immunoprecipitation. Frozen B5 fixed and formalin fixed, paraffin embedded tissue sections can be stained using anti-vimentin antibody. Mouse monoclonal anti-vimentin antibody reacts specifically with an epitope of the cytoskeletal protein vimentin (58kD). The product shows cross reactivity with vimentin from bovine, human, pig, sheep, cat, rat, mouse and rabbit.

Egyediség

Recognizes an epitope of the cytoskeleton protein vimentin that is not detected in human lymphoid cells or derived malignancies. It is therefore useful in differentiating sarcomas from lymphomas.

Immunogen

human thymic nuclear extract

Alkalmazás

Monoclonal Anti-Vimentin antibody can be used in immunocytochemistry and immunohistochemical staining. It can also be used in immunofluorescence staining (diluted 1: 500) .
Monoclonal Anti-Vimentin antibody produced in mouse has been used in western blotting and immunolabeling.

Biokémiai/fiziológiai hatások

Vimentin plays an important role in modulating cell growth. It plays a pivotal role in epithelial-mesenchymal transition. Vimentin acts as a potential target for anti-metastasis therapy for nasopharyngeal carcinoma (NPC). It regulates cellular architecture and tissue integrity. It is also involved in tumorigenesis and the metastatic spread of cancer. Vimentin provides stress resistance. It might be involved in neuritogenesis.

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Nem találja a megfelelő terméket?  

Próbálja ki a Termékválasztó eszköz. eszközt

javasolt

Product No.
Leírás
Árazás

Tárolási osztály kódja

12 - Non Combustible Liquids

WGK

nwg

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

Már rendelkezik ezzel a termékkel?

Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

Role of vimentin in early stages of neuritogenesis in cultured hippocampal neurons
Boyne LJ, et al.
International Journal of Developmental Neuroscience, 14(6), 739-748 (1996)
M J Gibson et al.
Endocrinology, 141(9), 3065-3071 (2000-08-31)
Axons of GnRH neurons terminate at the median eminence in the medial basal hypothalamus (MBH) of the brain early in development. Similarly, GnRH neurons in grafts of preoptic area (POA) tissue within the third ventricle of hypogonadal mice preferentially innervate
Vimentin is a crucial target for anti-metastasis therapy of nasopharyngeal carcinoma
Wang W, et al.
Molecular and Cellular Biochemistry, 438(1-2), 47-57 (2018)
Margit A Huber et al.
The Journal of clinical investigation, 114(4), 569-581 (2004-08-18)
The transcription factor NF-kappaB is activated in a range of human cancers and is thought to promote tumorigenesis, mainly due to its ability to protect transformed cells from apoptosis. To investigate the role of NF-kappaB in epithelial plasticity and metastasis
C Allison Stewart et al.
Molecular endocrinology (Baltimore, Md.), 27(9), 1442-1454 (2013-08-02)
Müllerian duct differentiation and development into the female reproductive tract is essential for fertility, but mechanisms regulating these processes are poorly understood. WNT signaling is critical for proper development of the female reproductive tract as evident by the phenotypes of
View All Related Papers

Cikkek

Improving Reproducibility: Best Practices for Antibodies

In the midst of beeping lab timers, presentations and grant deadlines, it is easy to take for granted the quality of lab reagents.

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

Lépjen kapcsolatba a szaktanácsadással