SAB4200716

Anti-Vimentin antibody, Mouse monoclonal

clone VIM-13.2, purified from hybridoma cell culture

• Szinonimák: Anti-F5H288
• UNSPSC kód: 12352203
• NACRES: NA.41
• klón: VIM-13.2, monoclonal
• application: IF; IHC
• faj reaktivitás: mouse, bovine, canine, rat, monkey, human, chicken
• technika/technikák: immunoblotting: 0.25-0.5 μg/mL using human foreskin fibroblast HS68 cell line extract; immunofluorescence: 1.25-2.5 μg/mL using human foreskin fibroblast HS68 cells; immunohistochemistry: suitable
• citations: 7

Tulajdonságok

• biológiai forrás: mouse
• Minőségi szint: 200
• antitest forma: purified from hybridoma cell culture
• antitest terméktípus: primary antibodies
• klón: VIM-13.2, monoclonal
• Forma: buffered aqueous solution
• molekulatömeg: ~58 kDa
• faj reaktivitás: mouse, bovine, canine, rat, monkey, human, chicken
• fejlettebb validálás: independent; Learn more about Antibody Enhanced Validation
• koncentráció: ~1.0 mg/mL
• technika/technikák: immunoblotting: 0.25-0.5 μg/mL using human foreskin fibroblast HS68 cell line extract; immunofluorescence: 1.25-2.5 μg/mL using human foreskin fibroblast HS68 cells; immunohistochemistry: suitable
• izotípus: IgM
• kiszállítva: dry ice
• tárolási hőmérséklet: −20°C
• célzott transzláció utáni módosítás: unmodified
• Géninformáció: bovine ... Vim(280955) ; chicken ... Vim(420519) ; dog ... Vim(477991) ; human ... VIM(7431) ; mouse ... Vim(22352) ; rat ... Vim(81818) ; rhesus monkey ... Vim(705289)

Leírás

Általános leírás

Anti-Vimentin antibody, Mouse monoclonal (mouse IgM isotype) is derived from the VIM-13.2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with human foreskin fibroblast extract. Vimentin is a type III intermediate filaments (IF) expressed in a wide range of cell types. including, pancreatic precursor cells, fibroblasts and sertoli cells. It is also expressed in trophoblastic giant cells, neuronal precursor cells, lung epithelial cells, renal tubular and stromal cells. Vimentin is also present in leucocytes, macrophages, neutrophils and mesangial cells.

Immunogén

human foreskin fibroblast extract

Alkalmazás

Anti-Vimentin antibody, Mouse monoclonal has been used in:

• western blotting
• indirect immunofluorescence
• immunohistochemistry

Biokémiai/fiziológiai hatások

Vimentin plays a significant role in supporting and anchoring the cellular position of organelles, such as nucleus, endoplasmic reticulum, mitochondria (either laterally or terminally). It helps to maintain the mesenchymal cells especially their shape, motility, survival during of mechanical stress. Epithelial cancers like prostate, breast, lung show an increased vimentin expression. Vimentin is also highly expressed in gastrointestinal tumors, central nervous system tumors and malignant melanoma.

Fizikai forma

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biztonsági adatok

• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: nwg
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable