The SygRNA™ synthetic RNA system recruits SpCas9 nuclease to specific genomic targets. The tracrRNA (trans-activating crRNA) binds to a crRNA (CRISPR RNA) and the resulting crRNA-tracrRNA complex guides Cas9 protein to crRNA-specified sites in the genome. SygRNA™ synthetic tracrRNA and crRNAs are optimized for use with Cas9 expressing of cell lines, Cas9 protein, or Cas9 mRNA and compatible with a variety of delivery methods such as microinjection, lipofection, and electroporation.
Alkalmazás
Functional Genomics/Transgenic Applications
Creation of gene knockouts
Creation of knock-in animals or cell lines with promoters, fusion tags or reporters integrated into endogenous genes
Tulajdonságok és előnyök
HPLC-purified SygRNA™ Cas9 tracrRNA oligo is optimized for use with a target-specific cRNA and a source of SpCas9 protein for genome editing applications.
Kapcsolódás
The SygRNA™ Cas9 tracrRNA oligo is used in conjunction with a target-specific crRNA and a source of Cas9 protein such as TGEN-CP-50UG, CAS9P, or CAS9MRNA to form active RNP complexes. Select predesigned unique crRNAs against human, mouse and rat genes at CRISPR search.
The compendium of RNA-binding proteins (RBPs) has been greatly expanded by the development of RNA-interactome capture (RIC). However, it remained unknown if the complement of RBPs changes in response to environmental perturbations and whether these rearrangements are important. To answer these
Combine guaranteed sgRNAs with our comprehensive range of CRISPR products and tools, including Cas9 and delivery reagents, for efficient genome modification with higher specificity.
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