TGEN-CNP
Cas9 Nickase Recombinant protein from Streptococcus pyogenes
lyophilized powder
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
12352200
NACRES:
NA.51
Forma
lyophilized powder
Minőségi szint
kiszerelés
pkg of 250 μg
pkg of 50 μg
kiszállítva
ambient
tárolási hőmérséklet
−70°C
Általános leírás
Recombinant Cas9 proteins (∼160 KD) are ready-to-use reagents for genome engineering experiments.
When combined with target-specific guide RNAs, Cas9 proteins will form ready-to-use nucleases suitable for transfection of cell cultures and for the accelerated development of genetically-modified animals via one-cell embryo injection.
When combined with target-specific guide RNAs, Cas9 proteins will form ready-to-use nucleases suitable for transfection of cell cultures and for the accelerated development of genetically-modified animals via one-cell embryo injection.
Tulajdonságok és előnyök
- Highly Specific
- Highly Active
- Ready-to-inject/transfect
Kiszerelés
The kit comes in two pack sizes:
- TGEN-CNP-50UG
- TGEN-CNP-250UG
Komponensek
Each kit consists of:
- one vial of Cas9 recombinant protein
- one vial containing 1 mL of 1x dilution buffer
- one vial containing 1mL of Nuclease free water with glycerol
Elv
CRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids.
Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB.
Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB.
Kapcsolódás
Use our CRISPR Selection Tool to order gRNA atsigma.com/crisprs
Know more about Cas9 Nickase Recombinant protein
Know more about Cas9 Nickase Recombinant protein
Fizikai forma
Lyophilized Recombinant_Sp_Cas9 protein
Elkészítési megjegyzés
It is recommended that the lyophilized Cas9 protein be resuspended in the nuclease-free water containing 50% glycerol that is provided in the kit to get the desired
concentration.
The solution should be allowed to incubate at room temperature (20−23 °C) for 10 minutes. Centrifuge immediately before use.
concentration.
The solution should be allowed to incubate at room temperature (20−23 °C) for 10 minutes. Centrifuge immediately before use.
Tárolási osztály kódja
10 - Combustible liquids
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Válasszon a legfrissebb verziók közül:
Analitikai tanúsítványok (COA)
Lot/Batch Number
Sajnos jelenleg COA nem áll rendelkezésre ehhez a termékhez online.
Ha segítségre van szüksége, lépjen velünk kapcsolatba Vevőszolgálat
Már rendelkezik ezzel a termékkel?
Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.
Lépjen kapcsolatba a szaktanácsadással