S4014

Spectinomycin dihydrochloride pentahydrate

powder, suitable for cell culture and plant cell culture, BioReagent

• Szinonimák: Spectinomycin
• Tapasztalati képlet (Hill-képlet): C₁₄H₂₄N₂O₇ · 2HCl · 5H₂O
• CAS-szám: 22189-32-8
• Molekulatömeg: 495.35
• EC-szám: 244-554-3
• MDL-szám: MFCD00150886
• UNSPSC kód: 12352207
• PubChem Substance ID: 24899581
• NACRES: NA.76

Tulajdonságok

• Terméknév: Spectinomycin dihydrochloride pentahydrate, powder, BioReagent, suitable for cell culture
• biológiai forrás: synthetic (inorganic)
• Minőségi szint: 200
• termékcsalád: BioReagent
• Forma: powder
• hatékonyság: ≥603 μg per mg
• tárolási körülmény: (Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.)
• technika/technikák: cell culture | mammalian: suitable; cell culture | plant: suitable
• szennyeződések: endotoxin, tested
• szín: white to pale buff
• oldhatóság: H₂O: soluble 50 mg/mL at
• antibiotikus hatásspektrum: Gram-negative bacteria; Gram-positive bacteria
• alkalmazás(ok): agriculture
• Hatásmechanizmus: protein synthesis | interferes
• tárolási hőmérséklet: 2-8°C
• SMILES string: O.O.O.O.O.Cl.Cl.CN[C@@H]1[C@H](O)[C@H](NC)[C@H]2O[C@]3(O)[C@@H](O[C@H](C)CC3=O)O[C@@H]2[C@H]1O
• InChI: 1S/C14H24N2O7.2ClH.5H2O/c1-5-4-6(17)14(20)13(21-5)22-12-10(19)7(15-2)9(18)8(16-3)11(12)23-14;;;;;;;/h5,7-13,15-16,18-20H,4H2,1-3H3;2*1H;5*1H2/t5-,7-,8+,9+,10+,11-,12-,13+,14+;;;;;;;/m1......./s1
• Nemzetközi kémiai azonosító kulcs: DCHJOVNPPSBWHK-UXXUFHFZSA-N

Leírás

Általános leírás

Chemical structure: aminoglycoside

Spectinomycin has generally been used for the treatment of gonorrhea in males, and a study conducted shows that spectinomycin dihydrochloride pentahydrate provides effective treatment against gonorrhea as well. Clinically, spectinomycin dihydrochloride pentahydrate is easier to use in injections as it shows higher solubility than spectinomycin.

Alkalmazás

Spectinomycin dihydrochloride pentahydrate has been used as a supplement in different types of media.

Spectinomycin is an aminocyclitol antibiotic derived from Streptomyces spectabilis. It has been shown to have a wide variety of uses, including treating acute gonorrheal urethritis and cervictis, to mark cell layers to monitor cell fate during leaf development, as a selection marker in plant related transformation systems for plant cells containing the marker gene Spcr, to study respiratory tract infections of cattle caused by Pasteurella multocida and Mannheimia haemolytica, and to generate plants deficient for the plastid-encoded RNA polymerase on MS-agar media. Spectinomycin also has use in amplification of low copy number plasmid carrying replicons. It is recommended for use in cell culture applications at 7.5-20 mg/L.

Biokémiai/fiziológiai hatások

Mode of Action: Spectinomycin acts by inhibiting protein synthesis and elongation by binding to the bacterial 30S ribosomal subunit and interfering with peptidyl tRNA translocation.

Mode of Resistance: A mutation in rpsE, the gene for ribosomal protein S5, prevents the binding of spectinomycin. Resistance is also conferred by the aminoglycoside-3′-adenyltransferase gene (aadA).

Antimicrobial spectrum: Spectinomycin acts against gram-negative and gram-positive bacteria.

Vigyázat

Solutions can be stored at 2-8°C for several weeks or at -20°C for more extended periods.

Elkészítési megjegyzés

Stock solutions should be prepared at 10 mg/mL in water and filter sterilized.

Egyéb megjegyzések

Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.

Biztonsági adatok

• Piktogramok: GHS07
• Figyelmeztetés: Warning
• Figyelmeztető mondatok: H315,H319,H335
• Óvintézkedésre vonatkozó mondatok: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Veszélyességi osztályok: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Célzott szervek: Respiratory system
• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 2
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves, type N95 (US)