S0692

Spectinomycin

Ready Made Solution, 100 mg/mL in DMSO/H₂O, 1:1

• Szinonimák: Actinospectacin
• MDL-szám: MFCD04041129
• UNSPSC kód: 51281602
• NACRES: NA.85

Tulajdonságok

• biológiai forrás: Streptomyces sp.
• Minőségi szint: 200
• Forma: solution
• koncentráció: 100 mg/mL in DMSO/H₂O, 1:1
• szín: colorless
• oldhatóság: DMSO/H₂O, 1:1: soluble 100 mg/mL
• antibiotikus hatásspektrum: Gram-negative bacteria; Gram-positive bacteria; mycobacteria
• Hatásmechanizmus: protein synthesis | interferes
• kiszállítva: wet ice
• tárolási hőmérséklet: −20°C

Leírás

Általános leírás

Chemical structure: aminoglycoside

Alkalmazás

Spectinomycin is an aminocyclitol antibiotic derived from Streptomyces spectabilis. It has been shown to have a wide variety of uses, including treating acute gonorrheal urethritis and cervictis, to mark cell layers to monitor cell fate during leaf development, as a selection marker in plant related transformation systems for plant cells containing the marker gene Spcr, to study respiratory tract infections of cattle caused by Pasteurella multocida and Mannheimia haemolytica, and to generate plants deficient for the plastid-encoded RNA polymerase on MS-agar media. Spectinomycin also has use in amplification of low copy number plasmid carrying replicons. It is recommended for use in cell culture applications at 7.5-20 mg/L.

Biokémiai/fiziológiai hatások

Mode of Action: Spectinomycin acts by inhibiting protein synthesis and elongation by binding to the bacterial 30S ribosomal subunit and interfering with peptidyl tRNA translocation.

Mode of Resistance: A mutation in rpsE, the gene for ribosomal protein S5, prevents the binding of spectinomycin. Resistance is also conferred by the aminoglycoside-3′-adenyltransferase gene (aadA).

Antimicrobial spectrum: Spectinomycin acts against gram-negative and gram-positive bacteria.

Vigyázat

Solutions can be stored at 2-8°C for several weeks or at -20°C for more extended periods.

Elkészítési megjegyzés

Stock solutions should be prepared at 10 mg/mL in water and 0.2 μm filtered.

Tárolás és stabilitás

Keep container tightly closed in a dry and well-ventilated place. Containers which are opened must be carefully resealed and kept upright to prevent leakage.Store under inert gas.

Egyéb megjegyzések

Keep container tightly closed in a dry and well-ventilated place. Containers which are opened must be carefully resealed and kept upright to prevent leakage. Store under inert gas

Biztonsági adatok

• Tárolási osztály kódja: 10 - Combustible liquids
• WGK: WGK 2
• Lobbanási pont (F): 201.0 °F - closed cup
• Lobbanási pont (C): 93.9 °C - closed cup
• Egyéni védőeszköz: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)