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R1383

Sigma-Aldrich

RPMI-1640 Medium

With ʟ-glutamine, without glucose and sodium bicarbonate, powder, suitable for cell culture

Szinonimák:

Roswell Park Memorial Institute 1640 medium

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About This Item

MDL-szám:
MFCD00217820
UNSPSC kód:
12352207
NACRES:
NA.75

product name

RPMI-1640 Medium, With L-glutamine, without glucose and sodium bicarbonate, powder, suitable for cell culture

form

powder

technika/technikák

cell culture | mammalian: suitable

alkatrészek

L-glutamine: yes
phenol red: yes
sodium pyruvate: no
HEPES: no
NaHCO3: no

kiszállítva

ambient

tárolási hőmérséklet

2-8°C

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Általános leírás

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

Alkalmazás

RPMI-1640 Medium has been used as a cell culture medium to culture human MCF-7 breast cancer cells and human castrate-resistant prostate cancer (CRPC) cell lines for various cell culture experiments. It has also been used to culture human islets to assess the oxidative stress response in primary human islets.

Mennyiség

Formulated to contain 8.4 grams of powder per liter of medium.

Feloldás

Supplement with 2.0 g/L sodium bicarbonate.

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kiegészítés

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Árazás

Tárolási osztály kódja

13 - Non Combustible Solids

WGK

WGK 1

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

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Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

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