P8920

Poly-L-lysine solution

0.1 % (w/v) in H₂O

• Szinonimák: 2-(4-Cyanoanilino)-4,6-dichloropyrimidine, 4-[(4,6-Dichloro-2-pyrimidinyl)amino]benzonitrile
• CAS-szám: 25988-63-0
• MDL-szám: MFCD00165424
• UNSPSC kód: 41116121
• eCl@ss: 32160406
• NACRES: NA.47

Tulajdonságok

• biológiai forrás: synthetic
• Minőségi szint: 200
• eltarthatósági idő: Expiry date on the label.
• molekulatömeg: 150,000-300,000
• koncentráció: 0.1 % (w/v) in H₂O
• szín: colorless
• alkalmazás(ok): hematology; histology
• tárolási hőmérséklet: room temp
• InChI: 1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1
• Nemzetközi kémiai azonosító kulcs: WBSCNDJQPKSPII-KKUMJFAQSA-N

Leírás

Alkalmazás

Poly-L-lysine solution has been used for the attachment of endothelial cell cross sections to UV-crosslinked PET membrane frame slides. Poly-L-lysine coated slides were used for the placement of tissue sections for immunohistochemical assay.

Poly-L-lysine polymers can be used in promoting cell adhesion to solid substrates, conjugation to methotrexate for increased drug transport, microencapsulation of islets, cell microencapsulation technology, microarray glass slide coating, and chromosomal preparations. Lower molecular weight poly-L-lysine (30,000-70,000) is less viscuous in solution, but higher molecular weight versions provide more attachment sites per molecule.

Biokémiai/fiziológiai hatások

Poly-L-lysine is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. When it is absorbed to the cell culture surface, poly-L-lysine functions to increase the number of positively charged sites available for cell binding. With cells that can digest poly-L-lysine, poly-D-lysine should be used as the attachment factor.

Komponensek

Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Elkészítési megjegyzés

This product is supplied as a 0.10% (w/v) solution in water. Coating slides with this solution should be followed with a 5 minute incubation and drying the slides at room temperature. Coated slides will be stable for one year if protected from dust.

Biztonsági adatok

• Piktogramok: GHS07,GHS09
• Figyelmeztetés: Warning
• Figyelmeztető mondatok: H315,H317,H319,H411
• Óvintézkedésre vonatkozó mondatok: P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338
• Veszélyességi osztályok: Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable