P1524

Poly-L-lysine hydrobromide

suitable for cell culture, Mol wt ≥300,000

• Szinonimák: L-Lysine homopolymer hydrobromide
• CAS-szám: 25988-63-0
• MDL-szám: MFCD00237230
• UNSPSC kód: 12352209
• eCl@ss: 32160406
• PubChem Substance ID: 24898211
• NACRES: NA.26

Tulajdonságok

• Termék neve: Poly-L-lysine hydrobromide, mol wt ≥300,000
• Forma: powder
• Minőségi szint: 200
• molekulatömeg: ≥300,000
• technika/technikák: cell culture | mammalian: suitable
• szín: white to off-white
• alkalmazás(ok): cell analysis
• tárolási hőmérséklet: −20°C
• SMILES string: Cl.NCCCCC(N)C(O)=O
• InChI: 1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1
• Nemzetközi kémiai azonosító kulcs: WBSCNDJQPKSPII-KKUMJFAQSA-N

Leírás

Alkalmazás

Poly-L-lysine polymers can be used in promoting cell adhesion to solid substrates, conjugation to methotrexate for increased drug transport, microencapsulation of islets, cell microencapsulation technology, microarray glass slide coating, and chromosomal preparations. It has also been used as a transfection agent for efficient labeling and to facilitate endocytosis. Lower molecular weight poly-L-lysine (30,000-70,000) is less viscuous in solution, but higher molecular weight versions provide more attachment sites per molecule.

Biokémiai/fiziológiai hatások

Poly-L-lysine is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. When it is absorbed to the cell culture surface, poly-L-lysine functions to increase the number of positively charged sites available for cell binding. With cells that can digest poly-L-lysine, poly-D-lysine should be used as the attachment factor.

Elkészítési megjegyzés

Poly-L-lysine hydrobromide has a molecular weight >300,000. To remove the HBr, dissolve this product in a neutral buffer and dialyze to remove the salts. None of the poly-L-lysine products have been exposed to trifluoroacetic acid and are dialyzed to remove any monomers, dimmers, or trimers, confirmed by thin layer chromatography. In general, to use this product as an attachment factor, add 50 mL of sterile tissue culture grade water to 5 mg of poly-lysine, and aseptically coat the surface with 1 mL per 25 cm² of solution. After 5 minutes, remove the solution through aspiration and thoroughly rinse the surface. Let dry for two hours before introducing cells and medium.

Analízis megjegyzés

Molecular weight based on viscosity and is also assayed by MALLS.

Egyéb megjegyzések

Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Jogi nyilatkozat

Sterile solutions are stable for up to 2 years when stored at 2-8°C. It should be stored desiccated at -20°C.

Biztonsági adatok

• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves, type N95 (US)