P9155

Poly-ʟ-Lysine Hydrobromide

synthetic, mol wt 30,000-70,000, powder, γ-irradiated, suitable for cell culture, BioXtra

• Szinonimák: PDL HBr
• CAS-szám: 25988-63-0
• MDL-szám: MFCD00237230
• UNSPSC kód: 12352202
• eCl@ss: 32160406
• PubChem Substance ID: 24899014
• NACRES: NA.75

Tulajdonságok

• product name: Poly-L-lysine hydrobromide, mol wt 30,000-70,000, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture
• biológiai forrás: synthetic (organic)
• Minőségi szint: 200
• sterilitás: γ-irradiated
• termékcsalád: BioXtra
• form: lyophilized powder
• molekulatömeg: 30,000-70,000
• kiszerelés: pkg of 5 mg
• technika/technikák: cell culture | mammalian: suitable
• felület bevonatolás: 4 μg/cm²
• oldhatóság: H₂O: soluble 50 mg/mL, clear, colorless
• kiszállítva: ambient
• tárolási hőmérséklet: −20°C
• SMILES string: Cl.NCCCCC(N)C(O)=O
• InChI: 1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1
• Nemzetközi kémiai azonosító kulcs: WBSCNDJQPKSPII-KKUMJFAQSA-N

Leírás

Általános leírás

Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties. Poly-L-lysine hydrobromide is a nonspecific attachment factor in promoting cell adhesion to the substratum.

Alkalmazás

Recommended as a cell culture substratum when using 0.5 ml of a 0.1 mg/ml solution to coat 25 cm². Optimal conditions for attachment must be determined for each cell line and application.
Poly-L-lysine hydrobromide has been used to adhere the dissociated cells of cortical tissues into the culture plates. It has also been used to treat slides, to fix samples on it for immunohistochemical analysis.

Biokémiai/fiziológiai hatások

Poly-L-lysine is employed as a coating substance to facilitate improved cell attachment to culture surfaces. Research indicates that plates coated with poly-L-lysine (PLL) exhibit an accelerated cell growth rate. When mesenchymal stem cells (MSCs) are cultured on PLL-coated substrates, it helps preserve their stemness properties and prevents senescence. PLL-coated plates promote enhanced MSC growth and up-regulation of genes associated with cell adhesion, differentiation, proliferation, and signaling. Furthermore, porous PLL-coated surfaces have been shown to enhance cell proliferation and support the osteogenic differentiation of MSCs.

Komponensek

Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Vigyázat

Sterile solutions are stable for up to 2 years when stored at 2-8°C. It should be stored desiccated at -20°C.

Analízis megjegyzés

Molecular weight based on viscosity. Also assayed by MALLS.

Egyéb megjegyzések

For additional technical information on polyamino acids please visit the Polyamino acid FAQ resource.

Biztonsági adatok

• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves, type N95 (US)