P7280

Poly-ᴅ-Lysine Hydrobromide

synthetic, mol wt 30,000-70,000, powder, γ-irradiated, suitable for cell culture, BioXtra

• Szinonimák: PDL
• Lineáris képlet: D-Lys-(D-Lys)_n-D-Lys · xHBr
• CAS-szám: 27964-99-4
• MDL-szám: MFCD00131934
• UNSPSC kód: 12352202
• PubChem Substance ID: 24898835
• NACRES: NA.75

Tulajdonságok

• product name: Poly-D-lysine hydrobromide, average mol wt 30,000-70,000, lyophilized powder, γ-irradiated, BioReagent, suitable for cell culture
• biológiai forrás: synthetic (organic)
• Minőségi szint: 200
• sterilitás: γ-irradiated
• termékcsalád: BioReagent; BioXtra
• form: lyophilized powder
• molekulatömeg: average mol wt 30,000-70,000
• kiszerelés: pkg of 5 X 5 mg; pkg of 5 mg
• koncentráció: 0.016—0.032 mmol lysine
• technika/technikák: cell culture | mammalian: suitable
• felület bevonatolás: 4 μg/cm²
• oldhatóság: H₂O: soluble 50 mg/mL, clear, colorless
• kiszállítva: ambient
• tárolási hőmérséklet: −20°C
• SMILES string: O=C(C)[C@@](NC)([H])CCCCN.[Br]
• InChI: 1S/C6H14N2O2.BrH/c7-4-2-1-3-5(8)6(9)10;/h5H,1-4,7-8H2,(H,9,10);1H
• Nemzetközi kémiai azonosító kulcs: MEXAGTSTSPYCEP-UHFFFAOYSA-N

Leírás

Általános leírás

Poly-D-Lysine Hydrobromide (PDL), a positively charged polymer consisting of lysine residues, where each lysine unit is associated with a hydrobromide molecule. This hydrobromide molecule gives the poly-D-lysine a unique characteristic, enabling it to form crystals and making it easily soluble in water-based solutions.

Alkalmazás

Poly d lysine hydrobromide coating has been used:

• In coating tissue culture plate wells and coverslips for immunofluorescence microscopy of primary neuronal culture to study neurological disorders associated with Zika virus infection.
• To coat the coverslips for enhancing the attachment and proliferation of the myenteric cells isolated from the rat intestines.
• In preparing surface of coverslips for cell attachment for studying the myenteric plexus of rat’s small intestine.

Poly-D-lysine polymers can be used in preparing surfaces for cell attachment. The D-lysine polymers can also be used with cells that digest poly-L-lysine polymers and cause an excessive uptake of L-lysine.

This product is recommended as a cell culture substratum when using 0.5 - 1.0 mL of a 0.1 mg/mL solution to coat 25 cm². Lower molecular weight versions of the product are less viscous, but high more molecular weight versions provide more attachment sites per molecule.

Biokémiai/fiziológiai hatások

Poly-D-lysine (PDL) hydrobromide is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. After absorption to the culture surface, poly-D-lysine increases the number of positively charged cell binding sites.

Tulajdonságok és előnyök

Sterilized using γ-irradiation

Komponensek

Poly-D-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-D-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the ß structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Vigyázat

Sterile solutions are stable for up to 2 years when stored at 2-8°C. It should be stored desiccated at -20°C.

Elkészítési megjegyzés

This product has a molecular weight of 30,000-70,000 and is cell culture tested and gamma-irradiated. To remove the HBr, dissolve this product in a neutral buffer and dialyze to remove the salts. In general, to use this product as an attachment factor, add 50 mL of sterile tissue culture grade water to 5 mg of poly-lysine, and aseptically coat the surface with 1 mL per 25 cm² of solution. After 5 minutes, remove the solution through aspiration and thoroughly rinse the surface. Let dry for two hours before introducing cells and medium.

Egyéb megjegyzések

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Biztonsági adatok

• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves, type N95 (US)