P7181

Polyethylene glycol solution

Hybri-Max^™, 50 % (w/v), average mol wt 1,450, 0.2 μm filtered, BioReagent, suitable for hybridoma

• Szinonimák: PEG Solution, Polyethylene Glycol
• MDL-szám: MFCD00081839
• UNSPSC kód: 12161902
• PubChem Substance ID: 24898829
• NACRES: NA.75

Tulajdonságok

• grade: Hybri-Max^™
• Minőségi szint: 200
• sterilitás: 0.2 μm filtered
• termékcsalád: BioReagent
• form: solution
• molekulatömeg: average mol wt 1,450
• koncentráció: 50 % (w/v)
• technika/technikák: cell culture | hybridoma: suitable
• szennyeződések: endotoxin, tested
• tárolási hőmérséklet: 2-8°C
• SMILES string: C(CO)O
• InChI: 1S/C2H6O2/c3-1-2-4/h3-4H,1-2H2
• Nemzetközi kémiai azonosító kulcs: LYCAIKOWRPUZTN-UHFFFAOYSA-N

Leírás

Általános leírás

Polyethylene glycol (PEG) is a polymer of ethylene oxide and water, obtained upon condensation.

Alkalmazás

Polyethylene glycol solution has been used in the hybridization of the following:

• irradiated camel fibroblast cells with A23 hamster cells
• mouse splenocytes with Sp2/0-Ag 14 mouse myeloma cells
• rabbit splenocytes and cell line 240E-1

Recommended for use in a normal fusion protocol requiring 50% PEG.

PEG is used as a fusogen to obtain hybridomas for monoclonal antibody production. Induces cell hybridization.

Biokémiai/fiziológiai hatások

Polyethylene glycol (PEG) is used in detergents, emulsifying agents, soaps, metal costing process, pharmaceutical preparations and cosmetics. PEG is commonly used as a lubricant. It′s application is also observed in chromatographic stationary phases. PEG prevents crystal formation in emulsions.

Kiszerelés

Packaged in sealed ampules under nitrogen.

Egyéb megjegyzések

Contains 50% (w/v) polyethylene glycol (Av. Mol. Wt. 1450) in DPBS without calcium.

Feloldás

Solution is ready-to-use. If a less concentrated solution is desired, dilute with sterile DPBS without calcium (D5773). Some precipitate may appear after being exposed to cooler, but should disappear as the solution warms. Solution may be frozen if desired but should first be aliquotted to avoid repeated freeze/thaw cycles.

Jogi információk

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Biztonsági adatok

• Tárolási osztály kódja: 10 - Combustible liquids
• WGK: WGK 1
• Lobbanási pont (F): 212.0 °F - closed cup
• Lobbanási pont (C): 100 °C - closed cup
• Egyéni védőeszköz: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter