N4645

4-Nitrophenyl phosphate disodium salt hexahydrate

powder, BioReagent, suitable for cell culture, ≥97%

• Szinonimák: p-Nitrophenyl phosphate disodium hexahydrate, Disodium 4-nitrophenyl phosphate hexahydrate, di-Sodium 4-nitrophenyl phosphate, pNPP disodium hexahydrate
• Lineáris képlet: O₂NC₆H₄OP(O)(ONa)₂ · 6H₂O
• CAS-szám: 333338-18-4
• Molekulatömeg: 371.14
• Beilstein: 5324661
• EC-szám: 224-246-5
• MDL-szám: MFCD00066288
• UNSPSC kód: 12352204
• PubChem Substance ID: 24897708
• NACRES: NA.75

Tulajdonságok

• biológiai forrás: synthetic (organic)
• Minőségi szint: 200
• termékcsalád: BioReagent
• Teszt: ≥97%
• Forma: powder
• technika/technikák: cell culture | mammalian: suitable
• oldhatóság: H₂O: 100 mg/mL
• tárolási hőmérséklet: −20°C
• SMILES string: O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1
• InChI: 1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2
• Nemzetközi kémiai azonosító kulcs: KAKKHKRHCKCAGH-UHFFFAOYSA-L

Leírás

Általános leírás

Chromogenic substrate for the determination of acid and alkaline phosphatases. The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm.

Alkalmazás

4-Nitrophenyl phosphate disodium salt hexahydrate has been used:

• as a component in diethanolamine buffer to detect alkaline phosphatase-conjugated antibodies in enzyme linked immunosorbent assay (ELISA)
• to treat adherent cells in adhesion assay
• as a phosphatase substrate in in vitro phosphatase assay in substrate buffer for ELISA

Biokémiai/fiziológiai hatások

p-Nitrophenyl phosphate (pNPP) is the substrate of choice for use with alkaline phosphatase in enzyme linked immunosorbent assay (ELISA) procedures. It is the substrate of choice in alkaline phosphatase systems due to its high sensitivity. This substrate produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. This product is tested for use in cell culture applications. 4-nitrophenyl phosphate (pNPP), a small-molecule substrate can be used to study on alkaline phosphatases, acid phosphatases etc.

Biztonsági adatok

• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves, type N95 (US)