Ugrás a tartalomra
Merck
Összes fotó(1)

Key Documents

View All Documentation

N1891

Sigma-Aldrich

p-Nitrophenyl phosphate

SIGMAFAST™, chromogenic, tablet

Szinonimák:

pNPP tablets

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)

About This Item

MDL-szám:
MFCD00284586
UNSPSC kód:
12352204
NACRES:
NA.83

product name

SIGMAFAST™ p-Nitrophenyl phosphate Tablets, tablet, To prepare 5 mL

Minőségi szint

200

form

tablet

oldhatóság

deionized water: soluble 5 mL

tárolási hőmérséklet

−20°C

Looking for similar products? Látogasson el ide Útmutató a termékösszehasonlításhoz

Alkalmazás

SIGMA FAST p-Nitrophenyl phosphate Tablets has been used:
  • to quantify non-secreted alkaline phosphatase to monitor differentiation of osteoblast-like cells (SaoS-2)
  • to stain murine preosteoblast MC3T3-E1 cells in alkaline phosphatase assay
  • as a substrate for ExtrAvidin® alkaline phosphatase to measure hemoglobin-bound α-synuclein using enzyme-linked immunosorbent assay (ELISA)
  • as a substrate for alkaline phosphatase conjugated antibodies for detection in ELISA of abirin toxin
Developed for use as a soluble substrate for the detection of alkaline phosphatase activity in Enzyme Immunoassays (EIA). pNPP is the substrate of choice in high sensitivity alkaline phosphatase-based EIA assays.One pNPP tablet and one Tris buffer tablet are dissolved in deionized water and provide a ready-to-use substrate. Each Sigma FAST pNPP tablet set yields a solution containing 1.0 mg/ml pNPP, 0.2 M Tris buffer. EIA applications using pNPP may be read in timed assays or stopped with 3N NaOH for delayed readings. SIGMAFAST™ require only the addition of distilled or deionized water to prepare an active substrate solution. Tablets are individually packaged in foil packets.

Feloldás

Each tablet set dissolved in 5 mL deionized water yields a ready-to-use buffered solution containing pNPP.

Jogi információk

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

Tárolási osztály kódja

11 - Combustible Solids

WGK

WGK 3

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

Már rendelkezik ezzel a termékkel?

Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

Ruklanthi de Alwis et al.
The Journal of infectious diseases, 219(2), 295-304 (2018-10-16)
Nontyphoidal Salmonella (NTS) organisms are a major cause of gastroenteritis and bacteremia, but little is known about maternally acquired immunity and natural exposure in infant populations residing in areas where NTS disease is highly endemic. We recruited 503 pregnant mothers
Zimu Wu et al.
International journal of clinical and experimental pathology, 12(5), 1757-1763 (2020-01-15)
Post-stroke induction of alpha-synuclein (AS), a neuronal protein implicated in the pathogenesis of Parkinson's disease (PD), has been demonstrated to induce secondary brain damage after cerebral ischemia. Therefore, understanding the expression and pathogenic modifications of AS is clinically meaningful for
Conall H Watson et al.
PLoS neglected tropical diseases, 11(7), e0005786-e0005786 (2017-07-21)
Fiji, an upper-middle income state in the Pacific Ocean, has experienced an increase in confirmed case notifications of enteric fever caused by Salmonella enterica serovar Typhi (S. Typhi). To characterize the epidemiology of typhoid exposure, we conducted a cross-sectional sero-epidemiological
Ronit Rosenfeld et al.
Nature communications, 12(1), 944-944 (2021-02-13)
The coronavirus disease 2019 (COVID-19) pandemic, caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), exhibits high levels of mortality and morbidity and has dramatic consequences on human life, sociality and global economy. Neutralizing antibodies constitute a highly promising
Lan Xu et al.
Experimental cell research, 360(2), 413-420 (2017-09-30)
The highly glycosylated bone sialoprotein (BSP) is an abundant non-collagenous phosphoprotein in bone which enhances osteoblast differentiation and new bone deposition in vitro and in vivo. However, the structural details of its different glycosylation linkages have not been well studied
View All Related Papers

Cikkek

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Protocols

ELISA Procedures

Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

Lépjen kapcsolatba a szaktanácsadással