M7257

α-Mannosidase from Canavalia ensiformis (Jack bean)

ammonium sulfate suspension, ≥15 units/mg protein (biuret)

• Szinonimák: α-D-Mannoside mannohydrolase
• CAS-szám: 9025-42-7
• Enzyme Commission szám: 3.2.1.24 (BRENDA, IUBMB)
• EC-szám: 232-794-1
• MDL-szám: MFCD00130495
• UNSPSC kód: 12352204
• NACRES: NA.54

Tulajdonságok

• biológiai forrás: Canavalia ensiformis
• Minőségi szint: 200
• Forma: ammonium sulfate suspension
• specifikus aktivitás: ≥15 units/mg protein (biuret)
• idegen aktivitás: β-galactosidase, β-N-acetylglucosaminidase, α-galactosidase and α-L-fucosidase <0.05%
• tárolási hőmérséklet: 2-8°C
• SMILES string: [Zn+2]
• InChI: 1S/Zn/q+2
• Nemzetközi kémiai azonosító kulcs: PTFCDOFLOPIGGS-UHFFFAOYSA-N

Leírás

Általános leírás

α−Mannosidase from Canavalia ensiformis is a tretamer composed of two subunits that each contain two components at 44 and 66 kDa.

α-Mannosidase is an exo-glycosidase and an inc metalloenzyme.

Lysosomal α-mannosidases are class II mannosidases that belong to glycoside hydrolase family 38 and play an important role in the degradation of asparagine-linked carbohydrates of glycoproteins.

Alkalmazás

α-Mannosidase can be used to liberate mannose from a variety of synthetic and natural α-mannosides. It has also been used in a study to investigate the causes of neurodegeneration in mucolipidosis II ′knock-in′ mice.

α-Mannosidase from Canavalia ensiformis (Jack bean) has been used:

• for the digestion of neutral glycans.
• to remove O-glycans from recombinant β-glucosidase
• for the inhibition of renal fibroblasts proliferation

Liberates mannose from a variety of synthetic and natural α-mannosides.

Biokémiai/fiziológiai hatások

α−Mannosidase has been shown to inhibit the proliferation of B-lymphocytes.

α-Mannosidase cleaves near α1−2-, α1−3- and α1−6-linked mannose residues.

α-Mannosidase is an acid hydrolase which is located in plant vacuoles and is thought to be involved with the turnover of N-linked glycoproteins.

Egység definíció

One unit will hydrolyze 1.0 μmole of p-nitrophenyl α-D-mannoside to p-nitrophenol and D-mannose per min at pH 4.5 at 25°C.

Fizikai forma

Suspension in 3.0 M (NH₄)₂SO₄ and 0.1 mM zinc acetate, pH 7.5

Biztonsági adatok

• Piktogramok: GHS08
• Figyelmeztetés: Danger
• Figyelmeztető mondatok: H334
• Óvintézkedésre vonatkozó mondatok: P261 - P284 - P501
• Veszélyességi osztályok: Resp. Sens. 1
• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves, type N95 (US)