M2786

3-Maleimidobenzoic acid N-hydroxysuccinimide ester

crystalline

• Szinonimák: MBS
• Tapasztalati képlet (Hill-képlet): C₁₅H₁₀N₂O₆
• CAS-szám: 58626-38-3
• Molekulatömeg: 314.25
• Beilstein: 1505254
• MDL-szám: MFCD00005514
• UNSPSC kód: 12352108
• PubChem Substance ID: 24896768
• NACRES: NA.22
• Forma: crystalline

Tulajdonságok

• Forma: crystalline
• Minőségi szint: 200
• reakcióalkalmasság: reagent type: cross-linking reagent
• mp: 175-177 °C (lit.)
• oldhatóság: DMSO: ≤20 mg/mL
• funkcionális csoport: NHS ester; maleimide
• tárolási hőmérséklet: −20°C
• SMILES string: O=C1CCC(N1OC(C2=CC=CC(N3C(C=CC3=O)=O)=C2)=O)=O
• InChI: 1S/C15H10N2O6/c18-11-4-5-12(19)16(11)10-3-1-2-9(8-10)15(22)23-17-13(20)6-7-14(17)21/h1-5,8H,6-7H2
• Nemzetközi kémiai azonosító kulcs: LLXVXPPXELIDGQ-UHFFFAOYSA-N

Leírás

Általános leírás

may require addition of solvent to coupling buffer to at least 5% to maintain solubility

Alkalmazás

3-Maleimidobenzoic acid N-hydroxysuccinimide ester (MBS) has been used as a protein cross-linker. It has been used for the stabilization of actin filaments in plant tissue samples.

3-Maleimidobenzoic acid N-hydroxysuccinimide ester has been used:

• in the fixation of pollen in Arabidopis
• as a component of Murashige and Skoog (MS) medium to stabilize F-actin in Eucalyptus root hairs
• in the fixation of F-actin labelled pollen tubes of Lilium davidii

Heterobifunctional crosslinking reagent reactive toward primary amine and sulfhydryl. Typically, coupled initially to molecules containing primary amines by amide bonds buffered at pH 7.0, followed by coupling to compounds containing sulfhydryl by thioether at same pH. Useful for preparation of enzyme immunoconjugates and hapten carrier molecule conjugates.

Biokémiai/fiziológiai hatások

3-Maleimidobenzoic acid N-hydroxysuccinimide is used as a fixative for the preservation of pollen tube F-actin distribution. It imposes less damage when used on pollen tubes.

Egyéb megjegyzések

The aryl maleimide is less stable than alkyl maleimide linkers.

Biztonsági adatok

• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable