M0503

Mitomycin C from Streptomyces caespitosus

powder, contains NaCl as solubilizer

• Tapasztalati képlet (Hill-képlet): C₁₅H₁₈N₄O₅
• CAS-szám: 50-07-7
• Molekulatömeg: 334.33
• Beilstein: 7231816
• MDL-szám: MFCD00078109
• UNSPSC kód: 12352207
• PubChem Substance ID: 24896555
• NACRES: NA.76

Tulajdonságok

• biológiai forrás: Streptomyces caespitosus
• Minőségi szint: 200
• Forma: powder
• tartalmaz: NaCl as solubilizer
• szín: gray; purple/blue
• oldhatóság: H₂O: 1 vial + 4 mL, clear to slightly hazy, blue to purple
• antibiotikus hatásspektrum: Gram-negative bacteria; Gram-positive bacteria
• Hatásmechanizmus: DNA synthesis | interferes
• tárolási hőmérséklet: 2-8°C
• SMILES string: [H][C@]12CN3C4=C([C@@H](COC(N)=O)[C@@]3(OC)[C@@]1([H])N2)C(=O)C(N)=C(C)C4=O
• InChI: 1S/C15H18N4O5/c1-5-9(16)12(21)8-6(4-24-14(17)22)15(23-2)13-7(18-13)3-19(15)10(8)11(5)20/h6-7,13,18H,3-4,16H2,1-2H3,(H2,17,22)/t6-,7+,13+,15-/m1/s1
• Nemzetközi kémiai azonosító kulcs: NWIBSHFKIJFRCO-WUDYKRTCSA-N

Leírás

Általános leírás

Chemical structure: aziridine

Alkalmazás

Mitomycin C is produced by a strain of actinomyces, Streptomyces caespitosus. It contains three anticancer moieties, quinine, urethane, and aziridine groups. It is used to generate mitotically inactive feeder cells in cell culture systems, such as the mitotically inactive fibroblasts used in embryonic stem cell systems.

Biokémiai/fiziológiai hatások

Mode of Action: This product is an alkylating agent that specifically targets the guanine nucleoside sequence 5′-CpG-3′. It inhibits DNA synthesis by covalently reacting with DNA, forming crosslinks between complementary strands of DNA. This interaction prevents separation of complementary DNA strands, inhibiting DNA replication.

Antimicrobial Spectrum: Mitomycin C has strong antitumor activity, especially against Ehrlich ascites tumor cells, and strong bactericidal action against gram-positive and gram-negative bacteria.

Vigyázat

This vial contains 2 mg Mitomycin C and 48 mg NaCl. Stock solutions should be filter sterilized and stored at 2-8°C in the dark. Solutions at pH 6-9 can be stored at 0-5°C for up to a week, but if a precipitate forms, a fresh solution should be prepared - the precipitated solution has been proven toxic to cells.

Elkészítési megjegyzés

Mitomycin C is soluble in water at .5 mg/mL, with a pH of 6.0-7.5. It undergoes rapid degradation in acidic solutions with pH<6, and is mostly likely to retain activity in solutions with a pH between 6-9.

Biztonsági adatok

• Piktogramok: GHS08,GHS07
• Figyelmeztetés: Warning
• Figyelmeztető mondatok: H302,H351
• Óvintézkedésre vonatkozó mondatok: P201 - P301 + P312 + P330 - P308 + P313
• Veszélyességi osztályok: Acute Tox. 4 Oral - Carc. 2
• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable