Catalog numbers L1668 and L8902 are suitable for cell culture assays and cell stimulation. The end user needs to design and optimize the assays. These products have not been specifically tested for intracellular cytokine staining assays. It is recommended to review the literature for further information.
Összes fotó(2)
Fontos dokumentumok
L8629
Lectin from Phaseolus vulgaris (red kidney bean)
Erythroagglutinin PHA-E
Szinonimák:
Phaseolus vulgaris agglutinin, PHA
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Méret kiválasztása
5 MG
70 900,00 Ft
70 900,00 Ft
Az elérhetőséggel kapcsolatos kérdésekkel kérjük, forduljon Vevőszolgálatunkhoz.
Összes fotó(2)
Méret kiválasztása
Nézet módosítása
5 MG
70 900,00 Ft
About This Item
UNSPSC kód:
12352202
NACRES:
NA.32
70 900,00 Ft
Az elérhetőséggel kapcsolatos kérdésekkel kérjük, forduljon Vevőszolgálatunkhoz.
Javasolt termékek
Minőségi szint
Forma
salt-free, lyophilized powder
hatékonyság
<10 μg per mL agglutination activity (with erythryocytes)
összetétel
Protein, ≥80% biuret
tárolási hőmérséklet
2-8°C
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Related Categories
Biokémiai/fiziológiai hatások
PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.
Minőség
Tested for leucoagglutination.
Elkészítési megjegyzés
Purified by a modification of the method of Leavitt.[1]
Analízis megjegyzés
Agglutination activity is expressed in μg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of either human erythrocytes (2% in phosphate buffered saline, pH 6.8) or human leukocytes (107 per mL in saline) after 1 hr incubation at 25 °C.
Tárolási osztály kódja
11 - Combustible Solids
WGK
WGK 3
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Egyéni védőeszköz
Eyeshields, Gloves, type N95 (US)
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Analitikai tanúsítványok (COA)
Lot/Batch Number
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R D Leavitt et al.
The Journal of biological chemistry, 252(9), 2961-2966 (1977-05-10)
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Cikkek
Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.
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Which PHA is more suitable for cell stimulation and intracellular cytokine staining assays?
1 answer-
Helpful?
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What do the designations -P, -L, -M, and -E represent in the names of PHA products?
1 answer-
Generally, PHA-P or PHA-L is utilized to stimulate lymphocytes, although PHA-M will also work, but not to the same degree. The most active form is PHA-L (the mitogenic concentration is < 5μg/ml). PHA, or Phytohemagglutinin, comprises two molecular species:
1. Erythroagglutinin (PHA-E), which demonstrates low mitogenic activity and high erythroagglutinating activity.
2. Leucoagglutinin (PHA-L), which possesses high mitogenic and leucoagglutinating activity, accompanied by very low erythroagglutinating activity.
PHA-P represents the protein form of PHA before the erythroagglutinin and leucoagglutinin components are separated and purified. PHA-M refers to the mucoprotein form of Phytohemagglutinin.
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