L1006

L-Lactic Dehydrogenase from bovine heart

Type XVII, buffered aqueous glycerol solution, ≥400 units/mg protein

• Szinonimák: Lactate, (S)-Lactate: NAD⁺ oxidoreductase, L-LDH, LAD, LD
• CAS-szám: 9001-60-9
• Enzyme Commission szám: 1.1.1.27 (BRENDA, IUBMB)
• EC-szám: 232-617-8
• MDL-szám: MFCD00131466
• UNSPSC kód: 12352204
• NACRES: NA.54

Tulajdonságok

• biológiai forrás: bovine heart
• Minőségi szint: 200
• típus: Type XVII
• Teszt: 5.0—15.0 mg protein/mL (biuret)
• form: buffered aqueous glycerol solution
• specifikus aktivitás: ≥400 units/mg protein
• molekulatömeg: 140 kDa
• gyártó/kereskedő neve: Sigma-Aldrich
• technika/technikák: cell based assay: suitable
• szennyeződések: <10 μg/mg protein free ammonium
• szín: white
• pH: 7.5
• kation nyomok: NH₄⁺: ≤10 μg/mg protein
• UniProt elérési szám: P19858; Q5E9B1
• alkalmazás(ok): life science and biopharma
• idegen aktivitás: glutamic-pyruvic transaminase and glutamic-oxalacetic transaminase ≤0.02%; pyruvate kinase, myokinase and α-glycerophosphate dehydrogenase ≤0.01%
• tárolási hőmérséklet: 2-8°C
• Géninformáció: cow ... LDHA(281274) , LDHB(281275)

Leírás

Általános leírás

Research area: Cell signaling. L-LacticDehydrogenase (LDH) is present in the cell cytoplasm. and is part of the glycolytic pathway. Lactate dehydrogenase (LDH) is a ubiquitous molecule found in plants, yeast, mammals, and microorganisms and is a member of the oxidoreductase family. It has five isozymes (LD₁ to LD₅) and this composition varies in different tissues. LD₁ is present at higher concentrations in the heart, kidneys, and erythrocytes. LD₅ is found in the liver and skeletal muscles.(2)

Alkalmazás

L-Lactic Dehydrogenase from bovine heart has been used in ATPase assay of R2 complex Rvb1p-Rvb2p, RecA protein and sarcoplasmic reticulum Ca²⁺-ATPase (SERCA).

Biokémiai/fiziológiai hatások

Variation in the levels of L-Lactic Dehydrogenase (LDH) isoform is a diagnostic marker for tissue damage. The levels of LDH is useful in detecting dairy cattle disease, Mastitis. Dye based purified LDH from bovine heart has analytical applications.Lactic dehydrogenase (LDH)plays an essential role in the glycolytic pathway where it is involved in the conversionof pyruvate to lactate using NAD⁺as a co-factor. It is considered an important molecule that can be used incancer therapy as it acts as a glycolytic inhibitor. Inhibition of LDH isassociated with blocking of aerobic glycolysis in the tumour cells.

Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.

Egység definíció

One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.

Fizikai forma

Solution in 50% glycerol containing 0.025 M potassium phosphate buffer, pH 7.5

Analízis megjegyzés

Protein determined by biuret

Biztonsági adatok

• Piktogramok: GHS08
• Figyelmeztetés: Danger
• Figyelmeztető mondatok: H334
• Óvintézkedésre vonatkozó mondatok: P261 - P284 - P501
• Veszélyességi osztályok: Resp. Sens. 1
• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 1