L2625

L-Lactic Dehydrogenase from bovine heart

Type III, ammonium sulfate suspension, ≥500 units/mg protein

• Szinonimák: Lactate, (S)-Lactate: NAD⁺ oxidoreductase, L-LDH, LAD, LD
• CAS-szám: 9001-60-9
• Enzyme Commission szám: 1.1.1.27 (BRENDA, IUBMB)
• EC-szám: 232-617-8
• MDL-szám: MFCD00131466
• UNSPSC kód: 12352204
• NACRES: NA.54

Tulajdonságok

• típus: Type III
• Minőségi szint: 200
• Forma: ammonium sulfate suspension
• specifikus aktivitás: ≥500 units/mg protein
• UniProt elérési szám: P19858; Q5E9B1
• idegen aktivitás: pyruvate kinase ≤0.03%
• tárolási hőmérséklet: 2-8°C
• Géninformáció: cow ... LDHA(281274) , LDHB(281275)

Leírás

Általános leírás

Research area: Cell signaling

Lactate dehydrogenase (LDH) is a cytoplasmic enzyme that belongs to the oxidoreductase’s family. It is ubiquitously expressed in body tissues but at high levels in muscle, liver, and kidney. LDH exists as tetramer and comprises of two subunits (H and M). The LDH of eukaryotes undergo active-site loop gating for their catalytic functionality. L-lactate, which is the major enantiomeric form of lactate in vertebrates, is produced by the L-isomers of LDH enzyme encoded by the LDHC gene.

Alkalmazás

L-Lactic Dehydrogenase from bovine heart has been used as a standard in cytotoxicity assay. It has also been used in glutamic pyruvic transaminase (GPT) assay.

Biokémiai/fiziológiai hatások

Lactate dehydrogenase (LDH) is a crucial enzyme that plays a vital role in the anaerobic metabolic pathway. It facilitates the conversion of lactate to pyruvate and NAD⁺ to NADH, and vice versa, within cells. This enzymatic reaction plays a crucial role in cellular energy production, particularly in environments with low oxygen levels or hypoxic conditions. LDH activity is also significant in tumor glycolysis and the Warburg effect. LDH is released into the bloodstream when the cells are damaged or undergo death. Therefore, LDH can be used as a potential marker of cell injury and death. High lactate dehydrogenase activity is associated with various medical conditions, such as liver disease, anemia, heart attack, bone fractures, muscle trauma, and certain types of cancer.

Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.

Egység definíció

One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.

Fizikai forma

Crystalline suspension in 2.1 M (NH₄)₂SO₄ solution, pH 6.0

Analízis megjegyzés

Protein determined by biuret

Biztonsági adatok

• Piktogramok: GHS08
• Figyelmeztetés: Danger
• Figyelmeztető mondatok: H334
• Óvintézkedésre vonatkozó mondatok: P261 - P284 - P501
• Veszélyességi osztályok: Resp. Sens. 1
• Tárolási osztály kódja: 10 - Combustible liquids
• WGK: WGK 1
• Egyéni védőeszköz: Eyeshields, Gloves, type N95 (US)