L2500

L-Lactic Dehydrogenase from rabbit muscle

Type II, ammonium sulfate suspension, 800-1,200 units/mg protein

• Szinonimák: Lactate, (S)-Lactate: NAD⁺ oxidoreductase, L-LDH, LAD, LD
• CAS-szám: 9001-60-9
• Enzyme Commission szám: 1.1.1.27 (BRENDA, IUBMB)
• EC-szám: 232-617-8
• MDL-szám: MFCD00131466
• UNSPSC kód: 12352204
• NACRES: NA.54

Tulajdonságok

• típus: Type II
• Minőségi szint: 200
• Forma: ammonium sulfate suspension
• specifikus aktivitás: 800-1,200 units/mg protein
• molekulatömeg: 140 kDa
• idegen aktivitás: pyruvate kinase, myokinase, malic dehydrogenase, glutamic-pyruvic transaminase, glutamic-oxalacetic transaminase and α-glycerophosphate dehydrogenase ≤0.01%
• kiszállítva: wet ice
• tárolási hőmérséklet: 2-8°C

Leírás

Általános leírás

Lactic Dehydrogenase (LDH) has a total molecular weight of 140 kDa and is composed of 4 subunits which are designated M subunit (muscle) and H subunit (heart). These subunits may be mixed in any of 5 combinations (M4, M3H1, M2H2, MH3, and H4). Skeletal muscle contains LDH that is predominately M4 with some small amounts of M3H and traces of H2H2. The H and M subunits are quite similar in molecular weight, but differ substantially in amino acid composition. Rabbit muscle LDH dissociates into dimeric species (MW = ~70 kDa) in acetate-chloride at pH 5.0, the dissociation is reversible. Biochemistry, 13, 3527-3531 (1974). Oxidizes glyoxylate and lactate.
Isoelectric point: 8.4-8.6
Optimal pH : 7.5 .

Alkalmazás

L-Lactic Dehydrogenase from rabbit muscle has been used:

• for protein-binding measurement
• to detect the action of oxaloacetate decarboxylase
• to determine serum L- and D-lactate

Biokémiai/fiziológiai hatások

Lactic dehydrogenase is responsible for the conversion of pyruvate to lactate in the fermentative metabolism.

Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.

Egység definíció

One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.

Fizikai forma

Crystalline suspension in 3.2 M (NH₄)₂SO₄ solution, pH 6.0

Analízis megjegyzés

Protein determined by biuret.

Biztonsági adatok

• Piktogramok: GHS08
• Figyelmeztetés: Danger
• Figyelmeztető mondatok: H334
• Óvintézkedésre vonatkozó mondatok: P261 - P284 - P501
• Veszélyességi osztályok: Resp. Sens. 1
• Tárolási osztály kódja: 10 - Combustible liquids
• WGK: WGK 1
• Egyéni védőeszköz: Eyeshields, Gloves, type N95 (US)