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Merck

I3146

Sigma-Aldrich

ITS Liquid Media Supplement (100×)

liquid, sterile-filtered, BioReagent, suitable for cell culture

Szinonimák:

Human ITS Liquid Media, Insulin-transferrin-sodium selenite

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez

Méret kiválasztása

5 ML
30 600,00 Ft

30 600,00 Ft


Az elérhetőséggel kapcsolatos kérdésekkel kérjük, forduljon Vevőszolgálatunkhoz.

Megrendelés igénylése nagy tételben

Méret kiválasztása

Nézet módosítása
5 ML
30 600,00 Ft

About This Item

UNSPSC kód:
12352205
NACRES:
NA.75

30 600,00 Ft


Az elérhetőséggel kapcsolatos kérdésekkel kérjük, forduljon Vevőszolgálatunkhoz.

Megrendelés igénylése nagy tételben

sterilitás

sterile-filtered

Minőségi szint

termékcsalád

BioReagent

Forma

liquid

technika/technikák

cell culture | mammalian: suitable
single cell analysis: suitable

szennyeződések

endotoxin, tested

kiszállítva

ambient

tárolási hőmérséklet

2-8°C

Alkalmazás

ITS Liquid Media Supplement (100×) has been used as a media supplement to culture:
  • α mouse liver 12 (AML-12) cells[1]
  • rat fetal testis explants[2]
  • human dental pulp stem cells (DPSC)[3]

Biokémiai/fiziológiai hatások

  • Insulin: a polypeptide hormone that promotes the uptake of glucose and amino acids and may owe an observed mitogenic effect to this property.[4]
  • Transferrin: an iron-transport protein.
  • Selenium: an essential trace element normally provided by serum.[5]

Komponensek

Contains 1.0 mg/ml recombinant human insulin, 0.55 mg/ml human transferrin (substantially iron-free), and 0.5 μg/ml sodium selenite at the 100x concentration.

Egyéb megjegyzések

ITS is a mixture of recombinant human insulin, human transferrin, and sodium selenite. It is a general cell supplement designed for use in non-complex media (e.g. MEM, RPMI-1640) and complex media (e.g. Ham?s F-12, DME/F-12, MEM) with sodium pyruvate.

Elkészítési megjegyzés

Prepared in Earle′s Balanced Salt Solution (EBSS) without phenol red.

Tárolási osztály kódja

12 - Non Combustible Liquids

WGK

WGK 1

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Egyéni védőeszköz

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Analitikai tanúsítványok (COA)

Lot/Batch Number

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Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

Christine M Reich et al.
Veterinary research communications, 36(2), 139-148 (2012-03-07)
In the dog, mesenchymal stem cells (MSCs) have been shown to reside in the bone marrow (bone marrow-derived mesenchymal stem cells: BM-MSCs) as well as in the adipose tissue (adipose tissue-derived stem cells: ADSCs). Potential application fields for these multipotent
Leonardo Ricotti et al.
Biomedical materials (Bristol, England), 6(3), 031001-031001 (2011-05-14)
Polyelectrolyte layer-by-layer (LbL) nanofilms are interesting polymeric structures, built by alternating adsorption of positively and negatively charged polyelectrolytes. They consist of multilayer sheets with nanometric overall thickness, and they can be used as supports and surface coatings for in vitro
Kelvin S C Cheung et al.
PloS one, 9(6), e98063-e98063 (2014-06-04)
MicroRNAs (miRs) play a pivotal role in a variety of biological processes including stem cell differentiation and function. Human foetal femur derived skeletal stem cells (SSCs) display enhanced proliferation and multipotential capacity indicating excellent potential as candidates for tissue engineering
Cyrill Géraud et al.
Hepatology (Baltimore, Md.), 52(1), 313-326 (2010-06-26)
Liver sinusoidal endothelium (LSEC) is a prime example of organ-specific microvascular differentiation and functions. Disease-associated capillarization of LSEC in vivo and dedifferentiation of LSEC in vitro indicate the importance of the hepatic microenvironment. To identify the LSEC-specific molecular differentiation program
Loraine L Y Chiu et al.
The Laryngoscope, 127(3), E91-E99 (2016-11-20)
Cartilage tissue engineering is a promising approach to provide suitable materials for nasal reconstruction; however, it typically requires large numbers of cells. We have previously shown that a small number of chondrocytes cultivated within a continuous flow bioreactor can elicit

Cikkek

Understand the role of selenium in cell culture media formulations. The Media Expert library explains how culture media ingredients support cell growth.

Understand how transferrin affects serum-free cell culture systems, crucial for biomanufacturing heterologous proteins like monoclonal antibodies.

Questions

1–3 of 3 Questions  
  1. What should you use to dilute this to a 1X solution?

    1 answer
    1. This product can be dissolved in a given target cell culture medium, e.g., non-complex media (MEM, RPMI-1640), to a 1X solution.

      Helpful?

  2. What are the product numbers for the stand alone components in I3146?

    1 answer
    1. The product numbers for the stand-alone components in I3146 are as follows:
      Insulin – I2643 or I3536 (sterile)
      Apo-Transferrin – T2252
      Sodium Selenite – S5261
      EBBS – E3024

      Helpful?

  3. The ITS product #I3146 has been on back order for a while. Is there an alternative with similar components?

    1 answer
    1. Based on the Media Supplement Formulation, the closest alternative to product No. I3146 is the I2521 ITS+1 Liquid Media Supplement (100×), which also contains BSA and Linoleic acid.

      Helpful?

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