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Merck
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I2521

Sigma-Aldrich

ITS+1 Liquid Media Supplement (100×)

liquid, sterile-filtered, BioReagent, suitable for cell culture

Szinonimák:

Insulin-transferrin-sodium selenite, linoleic-BSA supplement

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
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About This Item

UNSPSC kód:
12352205
NACRES:
NA.75

sterilitás

sterile-filtered

Minőségi szint

500

termékcsalád

BioReagent

Forma

liquid

technika/technikák

cell culture | mammalian: suitable

szennyeződések

endotoxin, tested

kiszállítva

ambient

tárolási hőmérséklet

2-8°C

Általános leírás

Serum-free culture environment avoids the variability and contaminants that can be introduced by serum. Serum-free formulations that substitute a purified form of the factors normally supplied by serum are suitable for many in vitro growth and differentiation studies. These factors include insulin, transferrin, selenium, pyruvate, and ethanolamine. The addition of other components varies greatly, depending on the cell type being studied and the basal medium employed. ITS is a mixture of insulin, human transferrin (partially iron-saturated), and sodium selenite. It is a general cell supplement designed for use in non-complex media (e.g., MEM, RPMI-1640) and complex media (e.g., Ham′s F-12, DME/F-12, MEM) with sodium pyruvate.

Alkalmazás

ITS+1 Liquid Media Supplement (100×) has been used as a supplement in high-glucose Dulbecco′s Modified Eagle Medium (DMEM) for differentiation of mouse mesenchymal stem cells (mMSCs) to chondrocytes. It has also been used as a supplement in basal differentiation medium for differentiation of human-induced pluripotent stem cell (hiPSC) into osteoblasts under serum-free conditions.
Insulin-transferrin-sodium selenite, linoleic-BSA has been used for serum-free cell culture applications.ITS+1 Liquid Media Supplement (100×) has been used in culturing cell lines and for the preparation of immortalisation medium for the production of cell lines from ′Immortomouse′ embryos.

Komponensek

Contains 1.0 mg/ml bovine insulin, 0.55 mg/ml human transferrin (substantially iron-free), 0.5 μg/ml sodium selenite, 50 mg/ml bovine serum albumin and 470 μg/ml linoleic acid at the 100x concentration.

Elkészítési megjegyzés

Prepared in Earle′s Balanced Salt Solution (EBSS) without phenol red.

Tárolási osztály kódja

10 - Combustible liquids

WGK

WGK 2

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Egyéni védőeszköz

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Válasszon a legfrissebb verziók közül:

Analitikai tanúsítványok (COA)

Lot/Batch Number
0000322305
SLCP1573
SLCF9930
SLBZ6017
SLBW6622

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Dokumentumtár megtekintése

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Nature protocols, 5(3), 550-560 (2010-03-06)
Unlike humans, mouse bone marrow-derived mesenchymal stem cells (MSCs) cannot be easily harvested by adherence to plastic owing to the contamination of cultures by hematopoietic cells. The design of the protocol described here is based on the phenomenon that compact
Jing Wen et al.
Scientific reports, 10(1), 2782-2782 (2020-02-19)
The developmental competence of IVM porcine oocytes is still low compared with that in their in vivo counterparts. Although many studies reported effects of glucose metabolism (GM) on oocyte nuclear maturation, few reported on cytoplasmic maturation. Previous studies could not
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Molecular nutrition & food research, e1801393-e1801393 (2019-06-07)
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